In this study, the influences of operating parameters on flat-membrane submerged filtrations of BSA solution were investigated. The operating parameters included aeration rate, suction pressure, membrane characteristics and module inclination angle. The experimental results were summarized as follows: (1) During the operation of single-liquid phase filtration of BSA solution, hydrophilic membrane has a higher flux than hydrophobic one; (2) The flux of 1600 membrane inclination is higher than that of 900 inclination at the same aeration rate; (3) The way of blocking of the membrane does not happen step by step from standard blocking, complete blocking, intermediate blocking to cake filtration, but occurs coincidently; (4) The change of feed concentrate does not influence the way of blocking; (5) Aeration can effectively reduce the filtration resistance of cake filtration according to the blocking mechanism analysis.