Euonymus laxiflorus Champ 以及 Paenibacillus sp. TKU042 所生產α-葡萄糖苷酶抑制劑與α-澱粉酶抑制劑之研究

The studies on α‐glucosidase and α‐amylase inhibitors from Euonymus laxiflorus Champ and Paenibacillus sp. TKU042

阮文邦(Van-Bon Nguyen)

指導教授 ： 王三郎

淡江大學/理學院/化學學系博士班/博士(2017年)

https://doi.org/10.6846/TKU.2017.00406

摘要

本研究將收集自越南得樂省之二十六種中草藥進行α-葡萄糖
苷酶及α-澱粉酶抑制活性之分析。結果顯示大丁黃之甲醇萃取物
具有最高抑制活性。自此大丁黃萃取物分離出十一種具抑制活性之
化合物。其中三種(1，10，11)為新化合物，兩種為已知結構但新
發現具有α-澱粉酶抑制活性之化合物(13，21)，以及已知之酚類
化合物（2，9，16，17，18，19)。其中六種化合物（1，2，9，
13，16，17)具有與阿卡波糖這種糖尿病用藥不相上下之抑制活
性。此外自台灣土壤所篩選超過六百株細菌當中，類芽孢桿菌
TKU042 具有最佳α - 葡萄糖苷酶抑制活性。此菌發酵營養液
（nutrient broth）所得抑制活性高於阿卡波糖且具耐熱及酸鹼安
定性，小鼠降血糖試驗結果亦經證實。這些結果顯示，大丁黃及類
芽孢桿菌TKU042 發酵液具有應用於控制糖尿病及減肥之類保健食
品之潛力。

關鍵字

抑制劑 ； 大丁黃 ； 糖尿病 ； 類芽孢桿菌 ； 微生物轉換

並列摘要

The present study was aimed at finding safe, natural and abundant source of α-glucosidase and α-amylase inhibitors (αGIs and αAIs). Twenty-six samples of medicinal plants were collected in the Dak Lak province of Vietnam and evaluated for αGIs and αAIs. Trunk bark extract from Euonymus laxiflorus Champ (ELC) was selected as the best source of these inhibitors. Eleven novel active compounds were successfully isolated from ELC. Five compounds (1, 10, 11, 13 and 21) were determined as new aAIs in which 3 inhibitors (1, 10 and 11) were identified as new compounds. Another 6 compounds (2, 9, 16, 17, 18, and 19) were confirmed as known phenolic compounds. Notably, 6 compounds (1, 2, 9, 13, 16 and 17) showed a potency of slightly higher or comparable inhibition to that of acarbose. Among more than 600 bacterial strains isolated from Taiwanese soils, Paenibacillus sp. TKU042 was selected as the best producer of aGIs. The supernatant of fermented nutrient broth (FNB) showed stronger inhibitory activities than acarbose. The FNB aGIs also showed high thermal and pH stability, and acceptable effect on reducing plasma glucose in mice. All of the results suggest that ELC and FNB could have potential use for type 2 diabetes and obesity treatments or health foods development.

並列關鍵字

Inhibitors ； Euonymus laxiflorus Champ ； diabetes ； Paenibacillus ； microbial conversion

參考文獻

22. Liang, T.W.; Tseng, S.C.; Wang, S.L. Production and characterization of antioxidant properties of

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