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  • 學位論文

生物分子分離的新穎方法

A Novel Approach to Separation of Biomolecules

指導教授 : 陳銘凱

摘要


利用帶有四碳鏈的膠體帶有芳香烴的膠體分離重組蛋白。以不同的進樣方向,配合不同鹽的濃度和停留在膠體的時間,找出並測試對樣品分離的前後順序條件。

關鍵字

四碳鏈 芳香烴 進樣方向

並列摘要


The use of colloidal gel with four-carbon chain and colloidal gel with aromatic hydrocarbon to separate recombinant proteins were studied. In a different direction of injection, with different salt concentration and residence time in the colloidal gel, the orders of separation of samples were tested and identified.

參考文獻


1. Juri Rappsilber and Matthias Mann.(2002) What does it mean to identify a protein in proteomics? TRENDS in Biochemical Sciences ; 27 : 74-78
2. Mitsuaki Yanagida.(2002) Functional proteomics; current achievements. Journal of Chromatography B: Biomedical Sciences and Applications ;771 : 89-106
3. Paul R. Graves and Timothy A. J. Haystead.(2002) Molecular Biologist's Guide to Proteomics. Microbiol Mol Biol Rev ; 66 : 39-63
5. Ming-Kai Chern,Wei-Jyh Shiah, Jyun-Jie Chen1, Tzung-You Tsai1, Hsin-Yin Lin, Chien-Wei Liu.(2009) Single-step protein purification by back flush in ion exchange chromatography. Analytical Biochemistry ; 392 : 174-176
7. 張慧蘭.淡江大學化學所碩士論文(2012)

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