啤酒酵母醛類去氫酶2和3，紫外光及過氧化物的影響

The effects of ultraviolet and peroxide on aldehyde dehydrogenase 2 and 3 from Saccharomyces cerevisiae

曾昱縉(Yu-Jin Zeng)

指導教授 ： 簡素芳 ； 陳銘凱

淡江大學/理學院/化學學系碩士班/碩士(2013年)

https://doi.org/10.6846/TKU.2013.00732

摘要

啤酒去氫酶 ALD2p 和 ALD3p 為 ACDH (acetaldehyde dehyrogenase) 家族中的成員，並利用 NAD+ 作為輔酶。文獻指出，在泛酸合成的途徑中， ALD2p 和 ALD3p 會將中間產物 3-aminopropanal 轉變成為 β-alanine。

在哺乳類中， ALDHs 存在於角膜蛋白質中，以保護眼睛，透過直接吸收紫外光、代謝脂質過氧化物、抗氧化的功能保護眼睛。

由於先前實驗測試不到 ALD2p 及 ALD3p 的活性，所以在此測試是否有其他的功能，例如上述的抗紫外光、代謝脂質過氧化物、抗氧化的功能。

透過剔除 ALD2、ALD3 基因及過度表現 ALD2、ALD3 基因，接著再用滅菌過的超純水清洗，稀釋成不同濃度將菌液沾點於固態培養基上，照射紫外光或稀釋成不同濃度沾點於含有 H2O2 的固態培養基上。

關鍵字

醛類去氫酶 2 ； 醛類去氫酶 3 ； 啤酒酵母菌

並列摘要

In Saccharomyces cerevisia ALD2 (YMR170c) and ALD3 (YMR169c) are the members of ACDH (acetaldydehyde dehydrogenase) family, and the gene products are supposed to use NAD+ as coenzyme. Recent reference also shows that ALD2 and ALD3 genes are require for pantothenic acid biosynthesis by conversion of 3-aminopropanal to β-alanine.

In mammals, ALDHs have been identified as corneal crystallins and to contribute to eye protection by directly absorbing ultraviolet, or detoxifying reactive aldehydes produced by lipid peroxidation to non-toxic metabolities and act as antioxidant.

In this study, we examined the effects of ultraviolet and peroxide by deletion and overexpression of ALD2 and ALD3 gene.

並列關鍵字

aldehyde dehydrogenase 2 ； aldehyde dehydrogenase 3 ； Saccharomyces cerevisiae

參考文獻

2. Karathia, H., Vilaprinyo, E., Sorribas, A., Alves, R. (2011). Saccharomyces cerevisiae as a Model Organism: A Comparative Study. Plos One 6, e16015.

4. Lindahl, R. (1992). Aldehyde dehydrogenases and their role in carcinogenesis. Critical Reviews in Biochemistry and Molecular 27, 283-335.

5. emize, F., Andrieu, E. and Dequin, S. (2000). Engineering of the Pyruvate Dehydrogenase Bypass in Saccharomyces cerevisiae: Role if the Cytosolic Mg2+ and Mitochondrial K+ Acetaldehyde Dehydrogenases Ald6p and Ald4p in Acetate Formation during Alcoholic Fermentation. Applied and Environmental Microbiology 66, 3151-3159.

6. Jacaobson, M. K. and Bernofsky, C. (1974). Mitochondrial acetaldehyde dehydrogenase from Saccharomyces cerevisiae. Biochemica et Biophysica Acta 350, 277-291.

7. Bostian, K. A. and Betts, G. F. (1978). Purification and Properties of Potassium-Activated Aldehyde Dehydrogenase from Saccharomyces cerevisiae. Biochemical Journal 173, 773-768.