Burkholderia cepacia TKU026 以烏賊軟骨粉為唯一碳/氮源發酵生產酪胺酸酶抑制劑之研究

The study on the tyrosinase inhibitor produced by Burkholderia cepacia TKU026 using squid pen as thesole carbon/nitrogen sources

許家豪(Chia-Hao Hsu)

指導教授 ： 王三郎

淡江大學/理學院/生命科學研究所碩士班/碩士(2010年)

https://doi.org/10.6846/TKU.2010.00597

摘要

本研究係以水產廢棄物為主要碳/氮源，經微生物發酵後其可生產酪胺酸酶抑制劑，並探討其較適培養條件為研究目的。使用菌株為Burkholderia cepacia TKU026，源自台灣北部土壤。
以50mL液態培養基含1％ 烏賊軟骨粉（squid pen powder；SPP）、0.1％ K2HPO4及0.05％ MgSO4．7H2O於250mL三角錐瓶，置放37℃、150rpm震盪培養三天可得較高酪胺酸酶抑制劑活性（5000U/mL）。
在抗菌實驗方面發現其發酵上清液在濃縮10倍及8倍時可有效抑制Aspergillus fumigatus及Fusarium oxysporum的生長。

關鍵字

酪胺酸酶抑制劑

並列摘要

The purpose of this study is utilized of marine wastes by microbes to produce tyrosinase inhibitor. To found the optimal conditions for production of tyrosinase inhibitor were investigated. Burkholderia cepacia TKU026 was isolated from the soil in northern of Taiwan.
The optimum culture condition for tyrosinase inhibitory production were found when the culture was shaken at 37℃ for 3 days in 50 mL and 100 mL medium containing 0.1% K2HPO4, 0.05% MgSO4．7H2O and 1% squid pen powder.
Both the culture supernatant concentrated 10 and 8 times showed inhibitory activities on Aspergillus fumigates and Fusarium oxysporum.

並列關鍵字

Burkholderia cepacia ； tyrosinase inhibitor

參考文獻

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