The purpose of this study is to examine the effects of integrating VR (virtual reality) into cooperative learning on seven grade students’ achievement in Biology and learning satisfaction. Quasi-experimental designs is adopted with four classes in a junior high school. Students were taught cell division in two weeks, using integrating VR into cooperative learning and traditional pedagogy. Four classes will be compared in terms of their learning achievement in Biology and learning satisfaction. The major findings of this study were summarized as follows. First, although the results indicated that there was no significant difference between the four classes on learning accomplishment in Biology, the statistics showed that the integration of VR has positive effects on students’ learning satisfactions, learners’ observational ability, and learners’ realization of the learning content. Finally, this study provided a clear picture for the future research of the integration VR of the cooperative learning.