The purpose of this study is to discuss various flapping angles effect on aerodynamic performance of micro aerial vehicle (MAV). We adopt a transmission system parameter as a criterion which has a successful remote flight with 367 seconds. By adjusting the length of links we can make different stroke angles. This changes result in different aerodynamic output, especially in lift and thrust force. Meanwhile, a lot of flapping signals from wind tunnel tests are acquired, this data help us realizes the relationship between lift and thrust force in various flapping angles. The flappers with four stroke angles are set and tested in the wind tunnel. The results show: Lift forces are increased with increasing inclined angle. From the experimental data, in order to find a successful flight parameter, the lift force must be over 8 gf and the thrust forces are still positive. The stroke angle of 39, 52.8, 70.6 degree are satisfied our need. At the same time, to compare and make sure the wind tunnel tests are correct, several MAV flight test are performed. The total weight of MAV is less than 8 grams, by controlling the tail angle, position of gravity center and the flapping frequency, we can control the velocity and inclined angle in flight. The flight test show the stroke angles of 39, 52,8 and 70.6 degrees are successful.