Serratia marcescens TKU011 生產 靈菌紅素之條件與定性

Production and characterization of prodigiosins from Serratia marcescens TKU011

王振宇(Chen-Yu Wang)

指導教授 ： 王三郎

淡江大學/理學院/生命科學研究所碩士班/碩士(2010年)

https://doi.org/10.6846/TKU.2010.00945

摘要

Serratia marcescens TKU011 係一株以蝦頭殼粉末作為唯一碳/氮源
之蛋白酶及幾丁聚醣酶生產菌。本研究發現以烏賊軟骨粉末（SPP）作
為碳/氮源時具有可生產紅色色素-靈菌紅素（prodigiosin）的能力，菌株
醱酵產生之紅色色素經分離純化後，藉由1H-NMR 及ESI-MS 確認合成
之靈菌紅素屬於prodigiosin。TKU011 生產靈菌紅素之較適培養為含有
1.5%烏賊軟骨粉末、0.1% K2HPO4 及0.05% MgSO4〃7H2O 之50 mL 液
態培養基（pH 7~9）於30℃振盪培養一天後接著移到25℃繼續培養兩
天，靈菌紅素產量可達0.978 mg/mL。醱酵上清液經試驗推測亦含有脂
胜肽類生物界面活性劑沙雷濕潤素（serrawettin），表面張力值隨著靈菌
紅素的產量增加而降低。

關鍵字

沙雷氏菌 ； 烏賊軟骨粉末 ； 靈菌紅素

並列摘要

A protease- and chitosanase-producing strain, Serratia marcescens TKU011, was cultivated by using shrimp-head powder（SHP）as the sole carbon/nitrogen source. In this study, a prodigiosin-like pigment was produced from TKU011 by using squid pen powder（SPP）as the sole carbon/nitrogen source. prodigiosin（PG）is one of prodigiosin-like pigments produced by S.marcescens TKU011 and was purified and characterized by1H-NMR and ESI-MS to determine its chemical structure and molecular weight. The optimized conditions for prodigiosin production were cells used to inoculate in 50mL of medium containing 1.5% SPP, 0.1% K2HPO4, 0.05% MgSO4．7H2O（pH7~9）shaken at 30℃ for one day and then cultivated at 25℃ for two days. The production of PG reached 0.978 mg/mL. Besides, TKU011 culture supernatant also contained the lipopeptide biosurfactant（serrawettin）, and the production of serrawettin increased with PG production.

並列關鍵字

Serratia marcescens ； squid pen powder ； prodigiosin

參考文獻

Anita, K., Mahnaz, M. A., and Fatemeh, A. F. 2006. Review of prodigiosin, pigmentation in Serratia marcescens. Journal of Biological Sciences, 6:1-13.

Alihosseini, F., Ju, K.S., Lango, J., Hammock , B.D., and Sun, G. 2008. Antibacterial colorants: characterization of prodiginines and their applications on textile materials. Biotechnology Progress, 24:742-747.

Bunting, M. I., Robinow, C. F., and Bunting, H. 1949. Factors affecting the elaboration of pigment and polysaccharide by Serratia marcescens. Journal of Bacteriology, 58:114-115.

Button, G. L., Miller, M. A., and Tsang, J. C. 1975. Antibiogram and lipid analysis of a pigmented strain of Serratia marcescens and its nonpigmented variants. Antimicrob. Agents Chemother, 7:219-222.

1958. Prodigiosin hydrochloride. Journal of Organic Chemistry ,23:1232-1233.