The aim of this study is to examine the gender differences in spatial cognitive processes. The research employed laboratory experiment. The space rotation recognition was used as the experimental task. The subjects were recruited from two types of sport school teams. The electroencephalograph was used to measure the subjects’ brain wave when they received the stimulus with different dimensional graphics. The study reveals that the average time spent by male in spatial recognition was less than that of female, but the average accuracy rate was lower than that of female. Moreover, the recognition of 3D graphics for male was more difficult than that of 2D graphics. For female, the average spending time and average accuracy rate in recognizing 3D graphics are higher than in 2D. There are significant differences in the correctness of spatial recognition in which sports the subjects are engaged. Male and female right brain parietal lobe for both 2D and 3D stimulus show P300 amplitude response.