防止HepG2肝癌細胞貼附之力學探討

A Mechanical Study on the anti-attachment of HepG2 Tumor Cells

徐智文(Chih-Wen Hsu)

指導教授 ： 楊龍杰

淡江大學/工學院/機械與機電工程學系碩士班/碩士(2011年)

https://doi.org/10.6846/TKU.2011.01024

摘要

癌細胞透過入侵循環系統而達到轉移之過程，與細胞的脫離以及貼附機制有關，為了探討此一現象，本研究分為靜態實驗與動態實驗兩部分。靜態實驗利用電子細胞基質阻抗判斷技術(Electric Cell-substrate Impedance Sensing, ECIS)製作阻抗感測晶片，使用戊二醛交聯之明膠微圖案，吸引細胞貼附於電極上方，並監測HepG2細胞之形態與貼附變化。而藉由每隔1小時所得到的細胞貼附面積與阻抗變化之關係，可成為動態實驗參考之依據。
動態實驗藉由PDMS微流道內壁之設計，改變細胞的貼附條件，觀察癌細胞欲入侵之前的貼附行為以及阻塞之可能，並將HepG2肝癌細胞注入微流道內，模擬癌細胞經過微血管之情形。利用RIE以電漿轟擊PDMS，改變微流道內壁之粗糙度，進而改變細胞貼附之能力。癌細胞進入微流道時所表現之不同狀況，可反應該環境對癌細胞透過循環系統達到轉移的成功與否，也表示微血管於某種狀況下，阻塞不通的情形，並探討癌細胞透過肝門靜脈進入肝臟時，癌細胞貼附以及阻塞之情形，進而協助癌症之預防及治療，如飲食、藥物治療、基因治療等研究。

關鍵字

轉移 ； ECIS ； 細胞貼附 ； 肝門靜脈

並列摘要

For metastasis of tumor cell invade into the circulatory system, it have to do with tumor-cell of detachment and attachment. This work presents the first part of a new framework for preventing the tumor-cell of carcinoma in situ transition from one organ to others. Using an ECIS (electric cell-substrate impedance sensing) chip coated with glutaraldehyde (GA)-crosslinked gelatin patterns suitable for cell attachment, the author monitor the cell adhesion situation not only by optical microscope but also by electrical means. This cell-culture experiment with 1-hour time resolution so far demonstrates that the attachment moment for HepG2 on gelatin surface is no longer than 4 hours after the cell dosing and these tumor cells cannot stay on GA-crosslinked gelatin surface for more than 7 hours.
The second part of experiment is dynamic. The author design an experiment and a microfluidic chip for investigating the relationship between the metastasis and the surface morphology of blood vessels. Using RIE (reactive ion etch) to change the inner wall morphology of PDMS. To different inner wall of PDMS roughness, the ability of cell adhesion is different. When tumor-cell through the microchannel, it may pass or get stuck. It’s mean that capillary may plugged by tumor-cell, or still smooth. Finally, to explore the adhesion of tumor-cell and blocking when tumor-cell into the liver through the hepatic portal vein. It can assist in the prevention and treatment of cancer, such as diet, drug, gene therapy research.

並列關鍵字

metastasis ； ECIS ； cell adhesion ； portal vein

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