具突張出口微流道之顆粒流動與應用

Particle Flow of a Microchannel with Abrupt Exit and Its Application

黃鼎翔(Huang Ting Hsiang)

指導教授 ： 楊龍杰

淡江大學/工學院/機械與機電工程學系碩士班/碩士(2018年)

https://doi.org/10.6846/TKU.2018.00960

摘要

本文針對具突張出口微流道之顆粒流動，使用 COMSOL Multiphysics的粒子流動計算模組，進行動態數值模擬，方便與2012年呂傑文之肝癌HepG2細胞灌流黏貼實驗結果進行比較。單一長直微流道寬15μm，高8μm，長500μm ，微顆粒大小1μm與5μm，流速1.39m/s，以水力直徑特徵長度依據，雷諾數範圍14.439。
純粹液體(水)模擬顯示，在雷諾數14.439，突張出口兩側出現渦漩迴流區，一般認為有利於微顆粒之黏貼。故使用粒子流動模組，依照(一)同時施放1000顆1μm粒子;(二)同時施放1000顆5μm 粒子;(三)同時施放粒徑1μm與5μm粒子各500顆;(四)分10次，每次施放100顆1μm粒子; (五) 分10次，每次施放100顆5μm粒子;(六) 分10次，每次施放每次施放1μm與5μm粒子各50顆，進行時間全長6000μs之動態數值模擬。
上述數值模擬與呂傑文實驗結果定性比較顯示:方式(二)、(五)的模擬較為逼真。
本文並製作聚二甲基矽氧烷微流道，進行方式(三)、(六)之灌流實驗與模擬比對，以明顯顆粒貼附外，分辨顆粒大小之能力。

關鍵字

生物晶片 ； 雷諾數 ； 渦旋 ； 微流道

並列摘要

This work used COMSOL Multiphysics particle flow module for dynamic numerical simulation of a microchannel with abrupt exit, which is convenient for comparison with Lu Jiewen's HepG2 cell perfusion and adhesion experiment results in 2012. The single long and straight microchannels are 15 μm wide, 8 μm high, 500 μm long, and the sizes of micro particle are 1 μm and 5 μm, and the flow velocity is 1.39 m/s. The characteristic length is based on the hydraulic diameter, and the Reynolds number is 14.439.

Pure liquid (water) simulations showed that at Reynolds number 14.439, vortex recirculation zones appeared on both sides of the outburst outlet, which is generally considered to be beneficial to the adhesion of microparticles. Therefore, the particle flow module is used in the following 6 methods of particle releasing: (1) to apply 1000 particles of 1μm at the same time; (2) to apply 1000 particles of 5μm at the same time; (3) to simultaneously apply 500 particles of particle size of 1μm and 5μm (triple) to 10 times 100 particles of 1μm each time; (4) 10 times; 100 particles of 1μm each time; (5) 10 times; 100 particles of 5μm each time, (6) 10 times; Fifty particles of 1 μm and 5 μm particles were each used to perform a dynamic numerical simulation of a total time of 6000 μs.

The qualitative comparison between the above numerical simulation and Lu Jiewen's experimental results shows that the simulations of methods (2) and (5) are more realistic.

In this work, fabrication process by polydimethylsiloxane(PDMS) microchannel were succeed, and the perfusion experiments and simulations of methods (3) and (6) were performed to check the situation of particle attachment and particle sorting capability.

並列關鍵字

Biochip ； Reynolds number ； Vortex ； microfluidic

參考文獻

參考文獻

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