6-薑醇對人類大腸癌細胞LoVo之抗癌機制研究

Investigating anticancer effects of 6-gingerol on human colon cancer cell LoVo

徐瑋佑(WEI-YU HSU)

指導教授 ： 高紹軒

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2010年)

https://doi.org/10.6834/CSMU.2010.00011

摘要

薑，是世界各地使用歷史悠久的香辛料與香味成份，因為具有治療腸胃不舒服、止吐、抗發炎以及抗癌等療效與生化功能也被當做中藥的成分。本篇論文針對薑的香味成份gingerols探討人類大腸直腸癌細胞LoVo之存活率。給予不同濃度的6-gingerol，培養不同時間，經MTT生長抑制試驗、流式細胞儀觀察PI染色後的細胞週期現象，分析是否有誘發細胞凋亡現象。結果顯示6-gingerol對LoVo有生長抑制以及細胞週期有G2/M arrest現象。Western blot檢測6-gingerol對細胞週期調節蛋白cyclinB1、Cdk1、 p27及p21表達的影響。6-gingerol作用24、48小時後可使LoVo細胞週期阻滯於G2/M期，並可抑制cyclinB1、 Cdk1蛋白的表現，促進p21、27蛋白的表現。結論：6-gingerol可以抑制LoVo增生，誘導其產生細胞週期停滯，且作用具有劑量依賴性，6-gingerol抑制LoVo增生的機制可能是使LoVo細胞週期停滯於G2/M期，阻止其通過G0/G1關卡。

關鍵字

細胞週期 ； 薑醇

並列摘要

Ginger, the rhizome of Zingiber officinale, known commonly as ginger, is consumed worldwide as a spice and a flavoring agent. It has been used in traditional medicine with carminative effect, anti-nausea, anti-inflammatory, and anti-carcinogenic properties.This study examined the growth inhibitory effects of the gingerols in human cancer cells. The effects of 6-gingerol on cell viability in human colorectal carcinoma LoVo cells were investigated. After the addition of different concentrations of 6-gingerol, the cultures were incubated further for various periods of time. Induction of apoptosis was manifested by cytotoxicity in MTT tests, cell cycle arrest in flow cytometry analysis. For the evaluation of apoptosis. The results indicated that 6-gingerol inhibited cell growth of LoVo, and G2/M arrest. The expression of cell cycle-related proteins, such as cyclin B1, Cdk1, p21 and p27 were detected by Western blot assay . Cell cycle of LoVo cell was arrested at G2/M phase after treatment of 6-gingerol for 24 and 48 h; Western blot assay showed that cyclin B1、Cdk1 protein was down-regulated and p21、27 protein was upregulated after 6-gingerol treatment. CONCLUSION: The 6-gingerol can inhibit the proliferation and induce cell cycle arrest to LoVo, in a dose-dependent manner. The possible mechanism to inhibit the proliferation of LoVo may be related with LoVo in G2/M phase due to 6-gingerol action to prevent LoVo to pass G0/G1 checkpoint.

並列關鍵字

Cell cycle ； gingerol

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