複方鯽魚萃取物對於以STZ誘發第2型糖尿病小鼠之影響

在第2型糖尿病中，由於胰島素阻抗使血糖更加控制不易，長期處於高血糖的情況下會誘導自由基大量生成，造成氧化壓力上升，而胰島素阻抗所造成的代謝障礙也會導致血脂異常，過量的葡萄糖則易走向多元醇路徑，不僅增加糖化壓力，最終會形成糖化終產物，引發糖尿病併發症。目前已有許多研究發現傳統中草藥具有降血糖、降血脂及抗氧化的效果。複方鯽魚萃取物是以鯽魚、山藥、枸杞、地黃等中藥材燉煮濃縮製造而成，本研究主要探討此複方鯽魚萃取物對第2型糖尿病小鼠的影響。實驗設計是將BALB/c小鼠以40 mg/kg的streptozotocin連續施打五天，誘發成第2型糖尿病(D)，再分別餵食複方鯽魚萃取物低劑量(6.25mg/kg/day；DL)及高劑量(31.25mg/kg/day；DH)四、八、十六週後犧牲，取其血液、脂肪組織、肝臟及腎臟進行分析。實驗結果發現複方鯽魚萃取物雖然沒有顯著的降血糖效果，但卻可以改善胰島素的阻抗情形，減少resistin基因表現。且透過抑制脂肪組織中diacylglycerol acyltransferase 1(DGAT-1)基因表現，減少脂質合成，降低血清膽固醇及三酸甘油酯含量；此外還可提升血清、肝臟、腎臟中抗氧化酵素的活性，減少脂質過氧化；而多元醇路徑中的關鍵酵素aldose reductase(AR)活性則顯著降低，果糖胺含量也有所減少。本研究結果支持複方鯽魚萃取物對於第2型糖尿病小鼠具有減少內臟脂肪堆積、降低血脂、增進胰島素敏感性、提升抗氧化能力及抑制葡萄糖走向多元醇路徑的效果。

關鍵字

鯽魚；胰島素阻抗；抗氧化； DGAT-1

並列摘要

Hyperglycemia induced abundant free radicals formation, results in increased oxidative stress. The metabolic disorders resulted from insulin resistance also leaded to dyslipidemia, and excessive glucose favors polyol pathway, and increases the generation of advanced glycation end-products (AGEs), which finally causes diabetes complications. The crucian carp complex formula extract includes Carassius auratus, Dioscorea opposite, Lycium barbarum, and Rehmannia glutinosa. The aim of this study was to investigate the effects of crucian carp complex formula extract on type 2 diabetes mice. Type 2 diabetes mellitus was induced by 40mg/kg STZ by injection into BALB/c mice for five days continuously. Then, mice were fed low or high doses of crucian carp complex formula extract for four, eight, and sixteen weeks. Results showed that crucian carp complex formula extract didn’t have hypoglycemic effect, but improved insulin resistance and decreased resistin gene expression. This extract inhibited DGAT-1gene expression in adipose tissue, reduced lipid synthesis, lowered serum cholesterol and triglyceride concentration. In addition, this extract also elevated the activity of antioxidant enzymes in serum, liver, and kidney, as well as reduced lipid peroxidation. The activity of aldose reductase, the key enzyme of polyol pathway, was lowered by this extract; and the concentration of fructosamine has also reduced. Therefore, these results supported that the crucian carp complex formula extract had the effects in reducing visceral fat accumulation, decreasing lipid, enhancing insulin sensitivity, elevating antioxidant capacity, and inhibiting glycation in type 2 diabetes mice.

並列關鍵字

Carassius auratus ； insulin resistance ； antioxidant ； DGAT-1

參考文獻

49. 倪智芩, 不同山藥降尿酸及保護腎臟功能的比較探討. 2008.

71. 行政院衛生署, <健康食品安全性評估方法>. 2010.

1. King, H., R.E. Aubert, and W.H. Herman, Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care, 1998. 21(9): p. 1414-31.

3. 美國糖尿病學會, Diagnosis and classification of diabetes mellitus. Diabetes Care, 2011. 34 Suppl 1: p. S62-9.

4. Fandrich, F. and H. Ungefroren, Customized cell-based treatment options to combat autoimmunity and restore beta-cell function in type 1 diabetes mellitus: current protocols and future perspectives. Adv Exp Med Biol, 2010. 654: p. 641-65.

國際替代計量

複方鯽魚萃取物對於以STZ誘發第2型糖尿病小鼠之影響

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