荷葉水萃物抑制動脈粥狀硬化機轉之探討

Studies on the effect and mechanisms of liquid extract of Nuciferine Leaf in inhibiting atherosclerosis

林櫻芳(Ying-Fang Lim)

指導教授 ： 王朝鐘

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2005年)

https://doi.org/10.6834/CSMU.2005.00019

摘要

動脈粥狀硬化最被接受的致病理論之ㄧ即是內皮功能失調（Endothelial dysfunction），例如：平滑肌細胞的遷移及增生，因此有效的抑制平滑肌細胞的遷移及增生將是一個防禦動脈粥狀硬化的主要方向。荷葉(Nuciferine Leaf)自古以來均記載著對於『活血化淤』具有強大的功效。「活血化淤」與現代預防動脈粥狀硬化的產生有著異曲同工之妙。目前荷葉的醫學功能並沒有深入研究。先前本實驗室的研究已顯示荷葉水萃物有抑制血管病變能力，本研究將探討荷葉對於預防動脈粥狀硬化機轉，利用抑制低密度脂蛋白氧化的實驗和使用平滑肌細胞進行抑制增生及位移的實驗。在抑制低密度脂蛋白氧化的實驗發現，在荷葉水萃物的處理之下，可抑制銅離子誘發低密度脂蛋白氧化、抑制Apo B蛋白的斷裂、抑制TBARS的生成及延緩Diene的生成，又透過Flow cytometry、MTT、DNA 斷裂的分析，發現荷葉水萃物可誘導增生的平滑肌細胞走向細胞凋亡路徑，也從一系列細胞凋亡的蛋白增加（如：Fas、Fas-L、Bax、Cyt C、Cap 3, 8, 9、p53、phosphorus-p53）證實荷葉水萃物的確促使增生的平滑肌細胞走向細胞凋亡的路徑，另外、從傷口癒合實驗、Gelatin zymography分析、Boyden chamber分析，亦發現荷葉水萃物有抑制平滑肌細胞遷移的能力。如此的結果顯示荷葉或許對未來心血管疾病的治療提供一個新的對策。

關鍵字

荷葉 ； 動脈粥狀硬化 ； 平滑肌細胞 ； 抗氧化 ； 降血脂 ； 細胞凋亡

並列摘要

Atherosclerosis is the major reason to cause these diseases. Based on the most acceptable theory is endothelial dysfunction represented as migration and proliferation in smooth muscle cell, the efficacious inhibition of migration and proliferation in smooth muscle cell is the major direction to preventing atherosclerosis. Traditionally, Nuciferine leaf was reported to possess powerful effect on decreasing serum lipids. It means that Nuciferine leaf also possess the same effect on preventing atherosclerosis. But upon to now, the researchers just emphasized the effects of Nuciferine leaf in clinical research but not in the mechanisms on preventing atherosclerosis. Because of that, we designed a series of experiments to study how Nuciferine leaf prevents atherosclerosis, and what were the mechanisms. The experimental results showed that Nuciferine leaf extract has efficacious inhibition on LDL- preoxidation-relative electrophoretic mobility, Apo B fragmentation, TBARS formation and diene formation. we used flow cytometry assay, MTT assay, DNA fragment assay and the result showed that Nuciferine leaf extract could induced apoptosis of the proliferated smooth muscle cell.and activated the caspase cascade and triggered apoptosis. Further wound healing, gelatin zymography and Boyden chamber study found that Nuciferine leaf extract could inhibited smooth muscle cell migration. According to the above, we suggested that Nuciferine leaf possessed the ability to reduce atherosclerosis via inhibiting the endothelial dysfunction. Furthermore, it had the potential to develop to a new health-caring herb to prevent atherosclerosis.

並列關鍵字

Nuciferine Leaf ； atherosclerosis ； smooth muscle cell ； antioxidation ； serum lipids ； apoptosis

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