- 學位論文
I.七層塔水萃物對四氯化碳誘導急性肝臟發炎影響機制之探討 II.人類微小病毒B19與博卡病毒結構蛋白獨立區域對呼吸道上皮細胞緊密連接之影響探討
I. Mechanisms of the Beneficial Effects of Ocimum gratissimum aqueous extract on rats with CCl4-induced acute liver injury II. Effects of Human Parvovirus B19 And Bocavirus VP1 Unique Region On Tight Junction Of Human Airway Epithelial A549 Cells
摘要
七層塔(Ocimum gratissimum)是一種常見的食物香料及傳統草藥，在早期曾經被用來治療各種疾病。為了進一步探討七層塔水溶性萃取物(OGAE)對肝臟的保護作用，我們利用了四氯化碳誘發雄性老鼠(male Wistar rats)產生肝臟損傷的動物模型，並餵食這些老鼠七層塔水溶性萃取物，探討七層塔水溶性萃取物是否有保護肝臟的效果。結果發現，七層塔水溶性萃取物可以提高損傷的肝臟產生清除自由基的酵素濃度及降低壓力性蛋白的產生，因此有機會成為進一步預防肝臟受損的健康輔助食品或草藥。 人類微小病毒B19(B19)及博卡病毒(HBoV)是重要的人類致病原。在人類微小病毒B19及博卡病毒上的結構蛋白獨立區域(VP1u)都具有表現類似分泌型磷脂水解酶A2(sPLA2)的酵素活性。然而，到目前為止少有文獻探討人類微小病毒B19及博卡病毒的VP1u結構蛋白獨立區域對細胞緊密連接(tight junction)的影響。因此，本研究主要藉由檢測磷脂水解酶A2(sPLA2)活性，上皮細胞穿透電阻(TEER)，以及西方墨點法來探討B19-VP1u及HBoV-VP1u是否會影響人類呼吸道上皮細胞A549細胞緊密連接(tight junction)。實驗結果指出，HBoV-VP1u比B19-VP1u對呼吸道細胞緊密連接(tight junction)的破壞扮演更重要的角色。
並列摘要
Ocimum gratissimum (OG) is known as a food spice and traditional herb, which has been recommended for the treatment of various diseases. To investigate the hepatoprotective effect of OG aqueous extract (OGAE), male Wistar rats challenged by carbon tetrachloride (CCl4) were used as the animal model of hepatic injury. Our data imply that OGAE can effi-ciently inhibit CCl4-induced liver injuries in rats and may therefore be a potential food or herb for preventing liver injuries. As is widely recognized, human parvovirus B19 (B19) and human bocavirus (HBoV) are important human pathogens. Obviously, both VP1 unique region (VP1u) of B19 and HBoV exhibit the secreted phospholi-pase A2 (sPLA2)-like enzymatic activity and are recognized to participate in the pathogenesis of lower respiratory tract illnesses. However exactly how, both VP1u from B19 and HBoV affect tight junction has seldom been addressed. Therefore, this study investigates how B19-VP1u and HBoV-VP1u may affect the tight junction of the airway epithelial A549 cells by examining phospholipase A2 activity and transepithelial electrical resistance (TEER) as well as performing immunoblotting analyses. The results suggest that HBoV-VP1u rather than B19 VP1u likely plays more important roles in the disruption of tight junction in the airway tract. Meanwhile, this discrepancy appears not to be associated with the se-creted phospholipase A2 (sPLA2)-like enzymatic activity.
參考文獻
延伸閱讀
- 陳宏銘（2006）。高壓氧氣治療內毒素誘發小鼠全身性發炎反應症候群模式的療效及細胞素影響與對肝臟保護效果之研究〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2006.02370
- 謝明儒（2011）。第一部分：以碳酸酐異構酶II、IX及XII RNA干擾技術探討對人類乳癌細胞侵襲及移動能力的影響第二部分：C型肝炎病毒套膜蛋白E2導致肝臟纖維化機制探討〔博士論文，中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834/CSMU.2011.00115
- 侯雅苓（2007）。人參皂苷萃取物及人參皂苷Rb1對四氯化碳誘發大鼠肝發炎反應及纖維化之影響〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1607200721331100
- 李燕霖（2005）。Inhibitory mechanism of quercetin and its metabolites on the growth of human hepatoma cells Hep 3B and the effects of quercetun on glutathione-related detoxification and antioxidation system of primary rat hepatocytes〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2005.01748
- Zhou, B. R., Zhang, J. A., Zhang, Q., Permatasari, F., Xu, Y., Wu, D., Yin, Z. Q., & Luo, D. (2014). Erratum to ＂Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1β, and Tumor Necrosis Factor-α via a NF-kB-Dependent Mechanism in HaCaT Keratinocytes＂. Mediators of Inflammation, (2014-5), 245-246. https://doi.org/10.1155/2014/513027