Zometa®和低强度镭射照射對成骨細胞的影響

Effects of Zometa® and Low Level Laser Irradiation on Osteoblasts

畢安卡(Katrina Bianca Baluyot Cruz)

指導教授 ： 黃裕峰

中山醫學大學/口腔醫學院/牙醫學系碩士班/碩士(2014年)

https://doi.org/10.6834/CSMU.2014.00050

摘要

Zometa是用來抑制骨頭吸收的雙磷酸鹽藥物(biphosphonates)，而近來文獻卻也指出它會降低骨生成的活性(osteoblastic activity)。相對於雙磷酸鹽藥物對細胞的影響，低能量雷射(low-level laser therapy, LLLT)的生物刺激也被利用於特定領域，來增強細胞的活性。 這篇體外實驗的目的是研究在不同劑量的Zometa暴露之下MC3T3-E1(mouse-derived calvarial cells)的藥物反應，以及同頻率及暴露量的低能量雷射之下骨生成細胞(osteoblastic cells)的生物刺激反應，其兩者之間的關係。 結論是雙磷酸鹽藥物的使用會降低成骨細胞增殖及遷移量，且特定時間內隨著Zometa濃度減少此效應也會弱化；然而透過低能量雷射的生物刺激，在特定時間內可以有效的增加細胞遷移和增殖(migration and proliferation)作用。 這個結果顯示，對於一定時間和濃度的雙磷酸鹽藥物暴露的骨生成細胞，雷射可能可以幫助抑制骨生成活性的降低。 未來我們還需要更多的研究來證實在骨再生(bone regeneration)的概念之下，低能量雷射療法對於Zometa確切且有限的影響成效。

關鍵字

Bisphosphonate ； Zoledronic Acid ； Low Level Laser Irradiation ； MC3T3-E1 osteoblast-like cell line

並列摘要

Zometa is a bisphosphonate therapeutic known to inhibit bone resorption had been discussed in the recent literatures to reduce osteoblastic activity. In contrast with its influences to cell processes, biostimulation of low level laser irradiation was utilized in certain applications to cause promotion of cellular activities. The purpose of this in vitro study was to investigate the response of MC3T3-E1 mouse-derived calvarial cells with the different concentrations of Zometa in relation to the osteoblastic cells that are biostimulated by different frequencies and exposure of low-level laser irradiation. As a result after application of dozes of the bisphosphonate therapeutic, it was observed that the reduction of osteoblastic proliferation and migration may seem to become limited as the influence of zometa weakens within the specified time. Whereas through the biostimulation by low level laser, cellular migration and proliferation was enhanced efficiently within the similar duration. Through this events, it can be suggested that laser irradiation may be of an aide to control in the reduction of osteoblastic activity during an amount of time and doze of incorporation of bisphosphonate with the osteoblastic cell culture. Further studies are needed to determine the definitive limitation potentials of Zometa in relation with the low-level laser therapy in the concept of bone regeneration.

並列關鍵字

Bisphosphonate ； Zoledronic Acid ； Low Level Laser Irradiation ； MC3T3-E1 osteoblast-like cell line.

參考文獻

1. Patntirapong, S., et al., Zoledronic acid suppresses mineralization through direct cytotoxicity and osteoblast differentiation inhibition. J Oral Pathol Med, 2012. 41(9): p. 713-20.

2. Du, X., et al., MS80, a novel sulfated polysaccharide, inhibits CD40-NF-kappaB pathway via targeting RIP2. Mol Cell Biochem, 2010. 337(1-2): p. 277-85.

3. Pyo, S.J., et al., Low-level laser therapy induces the expressions of BMP-2, osteocalcin, and TGF-beta1 in hypoxic-cultured human osteoblasts. Lasers Med Sci, 2013. 28(2): p. 543-50.

4. Koch, F.P., et al., The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression i vitro. Head Face Med, 2010. 6: p. 12.

5. Reid, I.R., et al., A single infusion of zoledronic acid produces sustained remissions in Paget disease: data to 6.5 years. J Bone Miner Res, 2011. 26(9): p. 2261-70.