以微型電腦斷層造影儀之影像及組織病理切片試驗 監測巴拉刈所導致大鼠肺纖維化之探討

Monitoring paraquat-induced pulmonary fibrosis in rats with micro-CT finding and histological examination

葉忠興(Chung-Hsin Yeh)

指導教授 ： 王凱淞

中山醫學大學/健康管理學院/公共衛生學系碩士班/碩士(2007年)

https://doi.org/10.6834/CSMU.2007.00031

摘要

本研究以微型電腦斷層造影儀之影像（這種不具有侵入性、不用犧牲動物、可長期性的監測同一動物，以減少個體差異方法）作為監測巴拉刈所導致之大鼠肺纖維化之情形。成鼠先秤重及Micro-CT造影，再以隨機方式分成控制、低劑量、中劑量、高劑量四組，依其組別給予單次不同劑量之巴拉刈水溶液，給藥後每週進行兩次秤重及Micro-CT造影，連續進行四週，並在每週造影後，每組隨機犧牲一隻成鼠，取出肺臟，進行檢體及組織病理觀察。結果顯示，控制組之肺臟檢體色澤正常，沒有出血，組織病理學則以I型肺泡細胞為主，無間質性肺炎且肺泡完整清淨，肺泡壁薄而完整；實驗組方面，第一~三週肺臟檢體外觀及組織病理的呈現出血點(petechia)及出血病灶且越趨嚴重，第四週則消退，且Micro- CT影像評估亦相同。各劑量之實驗鼠體重隨著巴拉刈劑量的增加，其體重增加量隨之減少，實驗鼠肺臟重量則因巴拉刈劑量的增加隨之增加，所以肺臟重量與體重之比值隨著劑量增加而升高，而經由三維的重建影像顯示隨著巴拉刈劑量的增加，肺部的出血及纖維化亦較為嚴重，因此肺部的有效容積量則逐漸降低。因此，此動物模式可做為診斷及監測肺纖維化之研究模式，而Micro-CT造影可做為診斷及監測的最佳工具之一。

關鍵字

微型電腦斷層造影儀 ； 巴拉刈 ； 肺纖維化 ； 組織病理學 ； 三維重建

並列摘要

This aim of study is monitoring Paraquat-induced pulmonary fibrosis in rats with Micro-computed tomography (Micro-CT) findings. That is non-invasion and do not need to sacrifice animals, and can supervise the same animals for reduced individual differences. Adult rats were measured body weight and imaged by Micro-CT, then divide into four groups randomly; according to control, low dosage, medium doagse, high dosage. Each group was given aqueous solution of Paraquat via oral single dosage at 0mg/kg, 40mg/kg, 60mg /kg, 80mg/kg, respectively.
In control group, the color and luster of the lung samples appeared normal and no petechia was observed. In histopathology sections showed that alveoli were complete and clear with walls being thin and brittle. In experimental group, some petechia were observed in lung samples , the area of fibroblast and fibrinogen in histopathology sections were found , and the ground-glass predominance and reticulation structure in Micro-CT findings were increased during the period from the 1st week to the 3rd week, and degrade in the 4th week after the administration. In the experimental group, the lung weights of adult rats were increased, with the dosage of paraquat was increased, but the body weight increments of adult rats were decreased, therefore the ratio of lung weights over body weights were increased with dosage, that was matched the results of associated researches.
The Mirco-CT images from 3D reconstruction show that the effective capacity volume of the lung gradually decreased with dosage increased, therefore Micro-CT as a powerful tool for fast diagnosis and longitudinal monitoring of pulmonary fibrosis. It might have more extensive application in the nearly future.

並列關鍵字

Micro-CT ； Paraquat ； Pulmonary fibrosis ； Histopathology ； 3D reconstruction

參考文獻

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