利用桿狀病毒表現系統表現人類微小病毒B19結構蛋白

Baculovirus Expression of Human Parvovirus B19 Structural Protein

許懷升(Huai-Sheng Hsu)

指導教授 ： 徐再靜

中山醫學大學/醫學院/免疫學研究所/碩士(2011年)

https://doi.org/10.6834/CSMU.2011.00041

摘要

人類微小病毒B19由VP1和VP2蛋白組成殼體，其中結構蛋白VP2（58kDa）佔病毒殼體組成的95%也是最主要的蛋白，而VP1（84 kDa）則只佔病毒殼體組成的5%。先前從國外文獻已知利用真核表達系統表達VP2殼體蛋白可產生能自行組裝之類病毒顆粒空殼體（VLP），其抗原性和免疫性都與B19病毒天然病毒顆粒相似。因此本研究利用桿狀病毒表現載體系統來表達B19病毒VP2殼體蛋白，並利用聚合酶連鎖反應、西方點墨法和間接免疫螢光法來分析確認，以期應用成為未來篩檢B19病毒感染及疫苗研究之平台。

關鍵字

人類微小病毒B19 ； 桿狀病毒

並列摘要

Previous studies have indicated that the capsids of human parvovirus B19 are composed of VP1 and VP2 proteins, also expression of VP2 alone in eukaryotic expression system could self-assemble into empty capsids, no matter in size, appearance, antigenicity or immunogenicity are similar to native virions. The empty capsids which without genetic materials are called virus-like particles (VLPs). We used Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus expressing B19 VP2 capsid proteins. Polymerase chain reaction, Western Blot and indirect immunofluorescence assay were used to identify the VP2 proteins in infected cells. We hope to employ the platform of VP2 proteins expression for B19 diagnosis and vaccine development in the future.

並列關鍵字

Human Parvovirus B19 ； Baculovirus

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