薑黃素衍生物HO-3867誘導人類口腔癌細胞凋亡的機制

The study of curcumin analog HO-3867 induces apoptosis in human oral cancer cells

陳彥霖(Yen-Lin Chen)

指導教授 ： 林巧雯

中山醫學大學/口腔醫學院/牙醫學系/碩士(2022年)

https://doi.org/10.6834/csmu202200064

摘要

在頭頸部的癌症中，口腔癌大約佔了百分之三十左右。時至今日，
雖然手術、放射線治療以及化學治療已經是口腔癌的標準治療，但也
因為口腔癌的復發率以及轉移造成對於口腔癌的治療以及預防都沒
有一個較理想的成果。薑黃 (Curcuma longa) 早期被人們用來做為香
料，染料等等功用，而其中的薑黃素 (curcumin) 被許多研究證實有
抗菌、抗氧化、抗發炎、保肝和低血糖保護作用。越來越多的證據表
明，薑黃素通過抑制腫瘤發生、進展、轉移、血管生成、化學抗性和
放射抗性在預防和治療多種人類癌症中發揮保護作用。而許多薑黃異
構物 (curcumin analog) 陸陸續續被研究證實可以改善薑黃生物吸收
率代謝率差的缺點。在本實驗中，我們觀察到薑黃異構物 HO-3867 可
以有效抑制 SCC-9 細胞存活率。而在流式細胞儀數據可以發現細胞
在 sub-G1 期百分比增加。此外，我們發現 HO-3867 藉由活化 caspase
8、caspase 9、caspase 3 和 PARP 的形成來誘導細胞凋亡，並且是透
過 JNK1/2 路徑誘導細胞凋亡。我們的研究證實 HO-3867 是一種有
效的抗癌劑，也相信藉由本實驗的結果可以幫助 HO-3867 在未來口
腔癌的治療或預防上可以有更多的運用。

關鍵字

口腔癌 ； JNK ； 薑黃異構物 ； HO-3867 ； 細胞凋亡

並列摘要

Oral cancer accounts for about 30 percent of head and neck cancers.
Although surgery, radiation therapy and chemotherapy are the standard
treatments for oral cancer, there is no ideal outcome for the treatment and
prevention of oral cancer due to the recurrence rate and metastasis of oral
cancer even today. Curcuma longa was previously used as a spice, dye, etc.
Many studies have confirmed that curcumin has antibacterial, antioxidant,
anti-inflammatory, hepatoprotective and hypoglycemic protective effects.
Accumulating evidence suggests that curcumin exerts protective effects in
the prevention and treatment of various human cancers by inhibiting
tumorigenesis, progression, metastasis, angiogenesis, chemoresistance,
and radio-resistance. Many curcumin analogs have been successively
confirmed to improve the shortcomings of turmeric's poor biological
absorption rate and metabolic rate. In this study, we observed that the
curcumin analog HO-3867 inhibited the survival of SCC-9 cells. We found
that HO-3867 effectively inhibited the growth of oral cancer cells. The flow
cytometry data indicate that HO-3867 induces the sub-G1 phase. Moreover,
we found that HO-3867 induces apoptosis by activating the formation of
caspase 3, 8, 9 and PARP. We also found that HO-3867 induces apoptosis
through the JNK1/2 pathway. Our results confirm that HO-3867 is an
effective anticancer agent, and we believe that the results of this
experiment can help HO-3867 to have more applications in the treatment
or prevention of oral cancer in the future.

並列關鍵字

Oral cancer ； Curcumin analog ； HO-3867 ； Apoptosis ； JNK

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