珍珠粉抗氧化性與延緩衰老之研究

Antioxidation and anti-aging effect of pearl powder

蕭夙君(Su-Chun Hsiao)

指導教授 ： 王進崑 ； 陳肅霖

中山醫學大學/健康管理學院/營養學研究所/碩士(2007年)

摘要

珍珠是傳統的中藥之一。含有多種胺基酸、礦物質。許多動物實驗結果指出，珍珠粉具有抗氧化、抗衰老、清除自由基等功效，而這些功效可能來自於珍珠粉中的蛋白質。
本研究，採用珍珠粉、珍珠粉蛋白質萃取物及去除蛋白質之珍珠粉進行抗氧化性、清除自由基之活性探討，結果顯示，珍珠粉與珍珠粉蛋白質萃取物皆具有抗氧化性、清除自由基的活性，並隨著劑量呈正相關：珍珠粉中的蛋白質可能是珍珠粉抗氧化之主要貢獻成分。
且以線蟲為模式探討珍珠粉之抗衰老作用，發現，珍珠粉之蛋白質萃取物可延長線蟲的壽命，具有抗衰老作用。
本研究目的在於了解服用珍珠粉後，人體之抗氧化性，本實驗採隨機、安慰劑對照之設計，共計19位受試者參與，實驗組之受試者每天服用珍珠粉3g，而對照組之受試者則每天服用安慰劑3g。本實驗共進行10週，試驗期為8週，試驗後2週為觀察期，試驗期間的第零、二、四、六、八、十週採集血液檢體。結果顯示，食用珍珠粉後，對體重、體脂肪及身體質量指數無顯著影響，在抗氧化性方面，可顯著增加受試者體內總抗氧化力、血漿含硫官能基及麩胱甘肽含量，顯著降低脂質之過氧化，顯著提高紅血球中麩胱甘肽過氧化酶及麩胱甘肽還原酶。此外，連續服用8週後，LDL氧化的時間亦顯著地由43分鐘延長至58分鐘。由本研究可知，每天服用珍珠粉確實可有效增加人體之抗氧化能力，並增加LDL之氧化耐受性，且可延長線蟲的壽命，應該具有抗衰老的作用，而蛋白質可能是主要的貢獻成分。

關鍵字

珍珠粉 ； 抗氧化性 ； LDL ； 自由基清除 ； 抗衰老

並列摘要

Pearl is one traditional Chinese medicine, which contains a lot of amino acids and minerals. Many animal studies indicate that pearl has antioxidation and anti-aging effect.
The study was focused on antioxidant effect and anti-aging ability of pearl powder. Results showed that both pearl powder and protein extract of pearl powder had antioxidation and scavenging efficiency on free radicals.
For anti-aging function, wild-type Caenorhabditis elegans was used. Protein extract of pearl powder effectively extended the life span. Protein was probably the main contribution of antioxidation for pearl. To further evaluate the effect of pearl powder on antioxidation in human body. The study employed a randomized, placebo-controlled design. Nineteen subjects were enrolled respectively in a ten-week trial. Each subject ate 3g of pearl powder capsule or placebo every day for continuous eight weeks. The total experimental period was ten weeks, including eight weeks of administration and two weeks of follow-up. Anthropometric measurements, blood collecting were performed at the initial, 2nd, 4th, 6th, 8th, 10th weeks of the testing period. Pearl powder greatly increased total antioxidant capacity, total thiol group levels, contents of glutathione, and reduced thiobarbituric acid reactive substance (TBARS) in plasma. The antioxidant enzyme activity in erythrocytes was also increased, including glutathione peroxidase and glutathione reductase. In additional, oxidative lag time of LDL was significantly increased from 43.50 to 58.00 mins.
In conclusion, pearl powder showed great antioxidation. The main contribution was probably from its protein.

並列關鍵字

Pearl powder ； antioxidation ； LDL ； anti-aging

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