電解還原水功能性評估

Functional Study of Electrolyzed-Reduced Water

蔡佳芳(Chia-Fang Tsai)

指導教授 ： 呂鋒洲

中山醫學大學/醫學院/醫學研究所/博士(2009年)

https://doi.org/10.6834/CSMU.2009.00055

摘要

第一部份
電解還原水具有高pH值、低氧化還原電位的功能性水。本研究先以超微量化學發光儀測量電解還原水抗超氧陰離子、氫氧自由基及過氧化氫等能力，並進一步探討電解還原水是否會增加對穀胱甘肽誘導血癌細胞(HL-60)進行細胞凋亡的表現。實驗結果發現電解還原水抗超氧陰離子、氫氧自由基及過氧化氫等三種自由基的能力相當於抗化劑，並經過不同濃度的穀胱甘肽處理後，發現電解還原水會幫助穀胱甘肽抑制血癌細胞HL-60增生，降低細胞的存活率，對於正常人類周邊血液單核細胞並未有任何影響。GSH與電解還原水處理6小時，藉由凝膠電泳分析觀察到DNA已經呈現片段化，同時藉由西方墨點法觀察電解還原水與GSH同時處理其蛋白質表現量的變化，結果顯示Bcl-2表現量減少、Bax蛋白質表現量增加、procaspase-3去活化，顯示電解還原水增加GSH誘發HL-60細胞產生細胞凋亡現象，應具有抗氧化劑的特性並作為輔助治療血癌藥物的潛力。
第二部份
本研究在探討電解還原水對四氯化碳誘發小鼠慢性肝炎的保護作用。實驗採用ICR小鼠並隨機分組為4組，分別為正常對照組、四氯化碳組、水飛薊素組、電解還原水組，除正常對照組外其他組每週管餵1 ml/kg四氯化碳(20% v/v)每星期投予兩次，共八星期。小鼠於四氯化碳投予前一週，即開始給予電解還原水飲用，直至實驗終了，為期二個月。電解還原水組於第六週、第八週對四氯化碳所升高的血清麩氨酸草酸轉氨酶、麩氨酸丙酮酸轉氨酶生化值有顯著降低作用。電解還原水組在第六週及第八週測得的膽固醇比四氯化碳控制組的值低。第八週所測得三酸甘油脂，和四氯化碳控制組比較後，電解還原水組於也呈現降低的數值。四氯化碳誘發慢性肝炎導致增加肝臟脾臟重量，但電解還原水可以緩減四氯化碳造成肝臟脾臟的重量增加。從病理結果可知電解還原水可緩解四氯化碳所造成的細胞壞死、膽管增生以及肝纖維化的程度。由這些結果顯示，電解還原水可以減輕四氯化碳所誘發的小鼠慢性肝損傷。

關鍵字

電解還原水 ； 活性氧物質 ； 人類血癌細胞 ； 細胞凋亡 ； 護肝 ； 四氯化碳

並列摘要

Part I
Electrolzyed-reduced water (ERW) is a higher pH and lower oxidation-reduction potential water. In the present study, we examined the enhanced effect of ERW in the apoptosis of leukemia cells (HL-60) induced by glutathione (GSH). An enhanced inhibitory effect on the viability of the HL-60 cells was observed after treatment with a combination of ERW with various concentrations of GSH, whereas no cytotoxic effect in normal peripheral blood mononuclear cells was observed. The results of apoptotic related protein indicated that the induction of HL-60 cell death was caused by the induction of apoptosis through upregulation of Bax and downregulation of Bcl-2. The results of further investigation showed a diminution of intracellular GSH levels in ERW and combination with GSH groups. These results suggest that ERW is an antioxidant, and that ERW, in combination with GSH, has an enhanced apoptosis-inducing effect on HL-60 cells, which might be mediated through the mitochondria-dependent pathway.
Part II
The study investigated the protective effect of electrolyzed reduced water (ERW) against carbon tetrachloride (CCl4)-induced liver damage. Male ICR mice were randomly divided into control, CCl4, CCl4 + silymarin, and CCl4 + ERW groups. CCl4-induced liver lesions included leukocytes infiltration, hepatocyte necrosis, ballooning degeneration, mitosis, calcification, fibrosis and an increase of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity. In addition, CCl4 also significantly decrease the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). By contrast, ERW or silymarin supplement significantly ameliorated the CCl4-induced liver lesions, lowered the serum levels of hepatic enzyme markers (ALT and AST) and increased the activities of SOD, catalase, and GSH in liver. Therefore, the results of this study show that ERW can be proposed to protect the liver against CCl4-induced oxidative damage in mice, and the hepatoprotective effect might be correlated with its antioxidant and free radical scavenging effect.

並列關鍵字

electrolyzed-reduced water ； Reactive oxygen species ； HL-60 cells ； apoptosis ； hepatoprotective ； carbon tetrachloride

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