- 學位論文
尿液與毛髮檢體中苯二氮平類藥物之質譜研究
Mass Spectrometric Analysis of Benzodiazepins In Urine and Hair
摘要
本研究成功建立九種常見的BZD類藥物，包含：Diazepam、Nordiazepam、Oxazepam、Lorazepam、Nitrazepam、Temazepam、Flunitrazepam、Alprazolam、Triazolam之毛髮與尿液質譜檢驗方法。在毛髮檢驗方面，主要採用高靈敏度的氣相層析負離子質譜(GC-NCI/MS)方法。研究發現，最低偵測極Flunitrazepm為3 pg/mg hair，其他藥物為1 pg/mg hair，而Temazepam可以低至0.1 pg/mg hair。在真實樣品分析方面，在戒治單位所採得的檢體，濃度大多高於70 pg/mg hair，而在服用BZD類藥物病人檢體方面，約為10 pg/mg hair。尿液確認檢測方法方面，採用氣相層析電子撞擊質譜方法(GC-EI/MS)，其最低偵測極限 Oxazepam、Lorazepam與Temazepam為0.1 ng/mL，其餘藥物為1 ng/mL。最後，我們評估發展液相質譜檢測方法之可行性，我們分別比較了ESI與APCI游離化方法，結果發現APCI在靈敏度方面上比ESI高約10-100倍，依序為APCI正離子＞APCI負離子≧ESI正離子＞ESI負離子。而在模擬尿液實驗中，因基質之干擾，使得APCI及ESI靈敏度降低約十倍左右。
並列摘要
This study was developed urine and hair testing for nine benzodiazepins, include Diazepam、Nordiazepam、Oxazepam、Lora zepam、Nitrazepam、Temazepam、Flunitrazepam、Alprazolam、Triazolam. The hair testing was used GC-NCI/MS. The limit of detection for Flunitrazepam was about 3 pg/mg hair, Temazepam was 0.1 pg/mg hair, and others were 1 pg/mg hair. The concentration of real sample from the treatment center was about 70 pg/mg, but it was quit different for the BZD drugs patients which were about 10 pg/mg. The technologies of hair testing were transferred to GC-EI/MS urine testing. The limit of detection for Oxazepam、Lorazepam and Temazepam were about 0.1 ng/mL. Others drugs was about 1 ng/mL. For LC/MS/MS, We compared the sensitivity of ESI and APCI. The result show APCI is better than ESI for 10-100 folds. The APCI Positive ion＞APCI negative ion ≧ESI Positive ion＞ESI negative ion. However, the real sensitivity of APCI and ESI were decrease 10 fold by urine matrix.