荷葉多酚藉由調節caveolin-1及iNOS氧化壓力作用抑制酒精性肝炎

The inhibitory effect of Nelumbo nucifera leaf polyphenol extracts on alcoholic hepatitis by regulating caveolin-1 and iNOS oxidative stress signals.

廖宥儒(Yu-Ju Liao)

指導教授 ： 王朝鐘

中山醫學大學/醫學院/生化微生物免疫研究所/碩士(2017年)

摘要

飲酒是造成肝炎主要的原因之一，肝臟長期暴露在有乙醇(ethanol)的環境也易引起氧化壓力現象(oxidative stress)引起肝癌。荷葉在過去已被證實具有抗氧化、降血脂、抑制肥胖等效果。一開始，在七週的酒精飼料餵食的動物模式下，發現荷葉多酚萃取物(Nelumbo nucifera leaf polyphenol extracts，NLPE)具有抑制酒精性肝炎(alcoholic hepatitis)的效果，為了更確定其詳細作用機制，利用細胞實驗探討NLPE可否通過cav-1的作用來保護慢性酒精引起的肝損傷，發現在酒精的誘導下cav-1會被活化並且阻止p-EGFR、p-ERK、p-STAT3所調控iNOS引起的氧化壓力現象來保護肝臟，而在NLPE的處理下cav-1與SOD-1有顯著增加的效果，基因剔除cav-1後加入荷葉p-EGFR、p-ERK、p-STAT3、iNOS的表現量則有些許回升的現象，此外cav-1的活化也抑制了細胞凋亡(apoptosis)、程序性壞死（necroptosis）的現象，實驗結果表明NLPE可以有效活化cav-1，且抑制了氧化壓力所調控細胞凋亡以及程序性壞死的現象，證實NLPE可以有效保護慢性酒精引起的肝損傷。

關鍵字

酒精性肝炎 ； 小窩蛋白 ； 氧化壓力 ； 荷葉多酚萃取物

並列摘要

Drinking is one of the main causes of hepatitis, liver long-term exposure to ethanol environment also cause oxidative stress and easier into liver cancer. Nelumbo nucifera in the past has been proven to have antioxidant, lipid-lowering, inhibiting obesity and other effects. In seven weeks of alcohol feed in animal models, we found that Nelumbo nucifera leaf polyphenol extracts (NLPE) had the effect of inhibiting alcoholic hepatitis. In order to determine its detailed mechanism of action, the use of cell experiments to explore whether NLPE through the role of cav-1 to protect chronic alcohol-induced liver injury, found that under the induction of alcohol cav-1 will be activated and prevent p-EGFR, p-ERK, p-STAT3 regulation INOS induced oxidative stress to protect the liver, while in the treatment of NLPE cav-1 and SOD-1 significantly increased the effect of gene exclusion cav-1 after the addition of lotus leaf p-EGFR, p-ERK, p-STAT3 , iNOS performance is somewhat The activation of cav-1 also inhibited the apoptosis and necroptosis. The results showed that NLPE could activate cav-1 and inhibit the apoptosis of cells by oxidative stress As well as the phenomenon of programmed necrosis, confirmed that NLPE can effectively protect chronic alcohol-induced liver injury.

並列關鍵字

Alcoholic liver disease ； caveolin-1 ； oxidative stress ； Nelumbo nucifera leaf polyphenol extracts

參考文獻

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