Nm23-H1及hTERT在子宮頸癌之表現

Human Nonmetastatic Clone 23 Type 1 and Human Telomerase Reverse Transcriptase in Cancer of Uterine Cervix

徐千剛(Chien-Gang Hsu)

指導教授 ： 柯俊良 ； 林隆堯

中山醫學大學/醫學院/醫學研究所/博士(2009年)

https://doi.org/10.6834/CSMU.2009.00078

摘要

Human nonmetastatic clone 23 (nm23-H1)和human telomerase reverse transcriptase (hTERT) 於高度子宮頸細胞內贅瘤 (high-grade cervical intraepithelial neoplasia) 及侵入性子宮頸癌 (invasive cervical cancer) 組織切片之免疫組織化學表現有增強趨勢，但是hTERT 免疫組織化學表現和子宮頸癌臨床病理特徵似乎較沒有關係，而nm23-H1 和子宮頸癌臨床病理特徵及預後存活似乎較有關係。我們因而以子宮頸組織晶片，研究nm23-H1免疫組織化學染色之表現，檢驗nm23-H1 蛋白表現和病人子宮頸癌臨床病理特徵、復發及預後存活之關係。我們並以real time polymerase chain reaction 驗證，於子宮頸癌組織，nm23-H1 之mRNA 表現較其周邊正常組織對照組強。我們進一步發現，nm23-H1 免疫蛋白之表現和子宮頸癌基質侵犯深度 (P = 0.003) 、腫瘤大小 (P = 0.044) 及細胞分化 (P = 0.025) 有關，且nm23-H1 免疫化學染色強的子宮頸癌病人預後較差。

關鍵字

子宮頸贅瘤

並列摘要

Human nonmetastatic clone 23 (nm23-H1) and human telomerase reverse
transcriptase (hTERT) may be separately involved in tumor progression of
uterine cervix. Moreover, we found normal cervix and cervical low-grade
intraepithelial neoplsia (low-grade CIN) samples had concurrent low expression
of hTERT and nm23-H1, whereas high-grade CIN [CIN2 (moderate dysplasia)
and CIN3 (severe dysplasia, carcinoma in situ)] SCC samples had concurrent
high immunoreactivity. However, hTERT immunoreactivity seemed to have no
significant association with clinicopathological variables of cervical cancer. On
the contrary, only deep stromal invasion was significantly associated with high
nm23-H1 immunoreactivity based on our limited cervical tissue samples. A high
cumulative recurrence hazard was demonstrated for the high nm23-H1
expression group. Therefore, using real time polymerase chain reaction and
tissue microarray of cervical samples, we further demonstrated that nm23-H1
mRNA expression and H score (median H scores: 2.0 vs. 0.3, P = 0.001) were
higher in cervical cancer tissues than normal counterparts, respectively.
Nm23-H1 expression was significantly associated with depth of stromal
invasion (P = 0.003), tumor diameter (P = 0.044) and cell differentiation (P =
0.025). Furthermore, positive nm23-H1 expression was significantly associated
with poor overall survival.

並列關鍵字

nm23-H1 ； hTERT

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