在倉鼠中八種益生菌降低血脂功效之研究

The investigation of the effect of eight probiotics on lowering serum lipids in hamster

徐智寅(Chih-Yin Hsu)

指導教授 ： 黃俊銘

中山醫學大學/健康管理學院/應用化學系碩士班/碩士(2010年)

摘要

益生菌已被認為具有降低血脂的功效。但並不是所有的益生菌都可以降低血脂。在本研究中八種益生菌包括五種乳酸桿菌 (lactobacilli) 分別為A菌 (嗜酸乳桿菌；Lactovacillus acidophilus)、C菌 (乾酪乳桿菌；Lactobacillus casei subsp)、LL菌 (乳球菌；Lactococcus lactis)、LP菌 (副乾酪乳桿菌；Lactobacillus pracasei)、LGG菌 (鼠李糖乳桿菌；Lactobacillus rhanosus GG) 和三種比菲德氏菌 (bifidobacteria) 分別為B菌 (雙叉雙歧桿菌；Bifidobacterium bifidum)、L菌 (長雙歧桿菌；Bifidobacterium longum)、I菌 (嬰兒歧桿菌；Bifidobacterium infantis) 被選定用來測試對於降低血脂的能力。實驗對象為雄性倉鼠，採餵食12%高油脂（添加0.2%的膽固醇）飼料，並同時給予不同劑量的益生菌 (200、400和 800 mg/kg)，8週後觀察血清生化指數的變化情形。結果顯示血清低密度脂蛋白膽固醇、血清總膽固醇以及血清三酸甘油酯的含量，高膽固醇的治療組 (400 mg/kg和800 mg/kg) 較高膽固醇的對照組有顯著降低，在治療組 (400 mg/kg) 的數值分別為117±79、302±196以及247±228，在治療組 (800 mg/kg) 的數值分別為93±31、255±89以及272±264，在高膽固醇的對照組分別為241±97、586±218以及546±303。因此，我們認為八種益生菌對於降低倉鼠體內血脂是有功效的。

關鍵字

益生菌 ； 高膽固醇血症 ； 倉鼠

並列摘要

Probiotics have been considered to be effective in the lowering lipid activity. But not all probiotics can reduce blood lipids. In this study, eight probiotics, including five lactobacilli were A strains (Lactobacillus acidophilus), C bacteria (Lactobacillus casei subsp), LL bacteria (Lactococcus lactis ), LP bacteria (Lactobacillus paracasei; Lactobacillus pracasei), LGG bacteria (Lactobacillus rhanosus GG) and three bifidobacteria were B strains (Bifidobacterium bifidum), L bacteria (Bifidobacterium longum), I bacteria (Bifidobacterium infantis), have been selected to test the ability to lower blood lipids. Male hamsters were fed diet with 12% high oil plus 0.2% cholesterol, and also given different doses of probiotics (200, 400 and 800 mg/kg). After 8 weeks, the variations in serum biochemical index were screen. The results showed that the levels of serum low-density lipoprotein cholesterol, serum total cholesterol and serum triglycerides in high cholesterol and probiotics (400 mg/kg and 800 mg/kg) -treated group were significantly lower than that in high cholesterol treated group. The high cholesterol and probiotics-treated group (400 mg/kg) values were 117 ± 79, 302 ± 196 and 247 ± 228, respectively; the high cholesterol and probiotics-treated group (800 mg/kg) values were 93 ± 31, 255 ± 89 and 272 ± 264, respectively; the high cholesterol-treated group were 241 ± 97, 586 ± 218 and 546 ± 303, respectively. Therefore, we suggest that the eight probiotics in reducing blood lipids in hamsters is effective.

並列關鍵字

probiotic ； hypercholesterolemic ； hamster

參考文獻

1.行政院衛生署。2010年。98年死因統計記者會新聞稿。

2.Toth PP. (2008). Subclinical atherosclerosis: what it is, what it means and what we can do about it. Int J Clin Pract 62, 1246-1254.

3.Cucina A., Scavo M. P., Muzzioli L., Coluccia P., Ceccarini S., Fuso A., and Cavallaro A. (2006). High density lipoproteins downregulate basic fibroblast growth factor production and release in minimally oxidated-LDL treated smooth muscle cells. Atherosclerosis 189, 303-309.

4.Frick M., Elo O., and Haapa K. (1987). Helsinki heart study: primary prevention trial with gemfibrozil in middle-age men with dyslipemia. N Engl J Med 317, 1237-1245.

5.Mann GV., Spoerry A. (1974). Studies of a surfactant and cholesteremia in the Maasai. Am J Clin Nutr 27, 464-469.