台灣人大腸直腸癌發生與淋巴球毒素α基因多型性之間的關係

Association of Polymorphism of The Lymphotoxin-αGene with Onset of Colorectal Cancer Cancer in Taiwanese Cohort

呂美純(Mei- Chun Lu)

指導教授 ： 許立松

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2011年)

https://doi.org/10.6834/CSMU.2011.00091

摘要

大腸直腸癌是一種常見的疾病，尤其在工業文明發展後，更是大量產生之癌化病變，根據統計僅次於肝癌及乳癌。一般認為致病原因可能與環境因子及基因多型性有關係。Lymphotoxins α﹙LTA﹚是一種細胞激素，可以調節免疫系統、參與發炎反應。本篇研究的主要目的在想了解在台灣族群中，LTA基因的基因多型性與大腸直腸癌發生之間有沒有關係。本實驗使用病例—控制試驗的方法，研究的病人數共有240人，其中106人為大腸直腸癌病患組；對照組為134人。我們利用分析PCR-NcoI-digested DNA 片段的方法來偵測LTA基因在intron 1 252位置的基因多型性。我們發現LTA 252 A→G 基因型態出現機率在病患組與對照組間是沒有差異的 (χ2 =1.56，P value = 0.212 ) 。由實驗結果我們可以推論：在台灣人族群，LTA基因在intron 1 252的基因多型性並不直接影響大腸直腸癌的好發性。

關鍵字

淋巴球毒素α

並列摘要

Colorectal cancer is one of the high prevalence malignancies all over the world. It is well accepted that multiple genetic alternations are involved in colorectal cancer formation. Lymphotoxins α (LTA) is a cytokine that can regulate the immune system involved in inflammation. Reports have demonstrated polymorphisms of LTA are associated with cancers formation, however, their role in colorectal cancer is still remain unclear. In this study, we investigate the relationship between polymorphism of LTA and colorectal cancer formation. PCR- restrict fragments leangth polymorphism (RFLP) method was employed to analyze LTA +252 A/G polymorphism. This study population consisted of 106 colorectal cancer patients and 134 control health peaples. No significant difference of +252 A/G of LTA was found between health control group and colorectal cancer patients(χ2 =1.56，P value = 0.212 ). In conclusion, the LTA polymorphism was not associated with colorectal cancer in Taiwan region.

並列關鍵字

tumor necrosis factor

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