鈦金屬表面形貌結合紅外光誘導塗層對生物活性的影響

Effect of bioactivity of surface topography and coating forming by infrared light-induced on titanium

林威廷(Wei-Ting Lin)

指導教授 ： 湯正明

中山醫學大學/口腔醫學院/口腔科學研究所/碩士(2018年)

摘要

磷酸鈣類的陶瓷具有良好的可吸收性。在生物組織中通常存在多種鈣離子和磷酸根離子，並且根據鈣磷比的不同，可分成不同種類。而常見的氫氧基磷灰石，是人體硬骨組織及牙齒表面的琺瑯質中的主要成分，具有良好的生物相容性及骨傳導性，常被應用在骨修復上。鈷離子作為缺氧誘導因子，可加快硬骨組織的再生。本研究先使用陽極氧化處理、鹼處理及酸處理在表面形成微米及奈米孔洞，並利用光線輔助仿生處理，分別再不同表面處理的試片上誘導磷酸鈣及鈷取代氫氧基磷灰石。結果發現，透過表面型態、晶體結構、表面化學性質，光線輔助仿生處理可在鈦試片表面上誘導磷酸鈣及鈷取代氫氧基磷灰石。而生物活性測試中，將試片浸泡SBF溶液後，表面的結晶性與浸泡前有明顯的變好，並且在試片表面發現有碳酸鈣的沉積。在細胞測試中，以人類骨母細胞株(MG-63)進行細胞存活率測試，結果表明大部分的試片經過光線輔助仿生處理過後，表面具有低的毒性。本研究以近紅外光誘導的塗層具有低毒性，所使用的紅外光線相較於脈衝雷射可提供更大的塗層生成面積，但由於塗層的鍵結強度較弱，導致在進行SBF浸泡時部分結晶解離於溶液中，未來可延長紅外光照射時間，以增加塗層的鍵結及厚度。

關鍵字

陽極氧化處理 ； 鹼處理 ； 酸處理 ； 光線輔助仿生 ； 鈷取代氫氧基磷灰石 ； 磷酸鈣 ； 生物相容性

並列摘要

Calcium phosphate (CaP) ceramics have good absorbability. There are usually many kinds of calcium ions and phosphate ions in biological tissues and can be classified into different types depending on the ratio of calcium to phosphorus. The hydroxyapatite is the main component in the human body tissue and enamel of the tooth surface. It has good biocompatibility and osteoconductivity and is often applied to bone repair. Cobalt ion acts as a hypoxia-inducible factor-1α, which accelerates the regeneration of hard bone tissue. In our study, micron and nanopores were formed on the surface by anodic oxidation treatmeant, alkali treatment, acid treatment and light-assisted biomimetic was used to induce CaP and cobalt-substituted hydroxyapatite (Co-HA) on different surface-treated specimens. It was found that LAB can induce CaP and Co-HA on the surface of titanium test pieces through the surface type, crystal structure and surface chemical properties. In the biological activity test, after the test piece was soaked in the SBF solution, the crystallinity of the surface was significantly improved before soaking and the deposition of CaP was found on the surface of the test piece. In the biocompatiability evaluation, cell viability was tested with the human osteosarcoma cells. The infrared light induced coating of this study has low toxicity and the infrared light used can provide a larger area for coating formation than pulsed laser, due to the weak bonding strength of the coating, SBF soaking is performed. When the partial crystals dissociate from the solution, the infrared light irradiation time may be prolonged in the future to increase the bonding and thickness of the coating.

並列關鍵字

Anodic treatment ； Alkali treatment ； Acid treatment ； Light-assisted biomimetic ； Cobalt-substituted hydroxyapatite ； Calcium phosphate ； Biocompatibility

參考文獻

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