脆江蘺鮮萃品及乾燥品在高脂肪誘導 倉鼠肥胖模式中降低血脂及體脂形成

The fresh extract and dried extract of Gracilaria burse-pastoris decrease lipidemia and formation of body fat in high fat-induced obese hamster

戴碧真(Pi-Chen Di)

指導教授 ： 李彗禎

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2012年)

https://doi.org/10.6834/CSMU.2012.00111

摘要

代謝症候群(metabolic syndrome)的出現代表潛藏的致病因素，在臨床上它常伴隨著心血管疾病的高度危險性，與腦血管疾病、心臟病、糖尿病以及高血壓等慢性疾病密切相關。衛生署於2011年公佈的國人十大死因中，有6項與代謝症候群有關。已知高血脂造成內臟脂質的堆積是造成代謝症候群的主因，而脂肪過度堆積在體內會造成肥胖，因此發展一無副作用並具降低肝臟或體脂肪效力的藥物或天然萃取物，儼然成為目前預防或治療代謝症候群及肥胖的研究目標。海藻植物脆江蘺(Gracilaria burse-pastoris)是目前國內產量最大的藻類，屬於紅藻的一種，部分臨床報告中證實紅藻具有調節脂質代謝的作用，但是對於脆江蘺調節脂質代謝作用的機制並不是很清楚。因此本研究中欲證實脆江蘺鮮萃品(GF)及乾燥品(GD)於肥胖動物模式誘導下，其減少血脂與減少脂質沉積的效用為何。在動物模式中，添加10% corn oil和2% cholesterol以誘導動物肥胖，為期八周，觀察各實驗組肝功能(ALT、AST)、血脂值(TCHO、TG、HDL、LDL)的變化，並測量動物肝臟與睪丸及腎周邊脂肪組織的重量。在動物模式中，初步的結果無法觀察到GF或GD對於血清脂質生成的抑制具有顯著性的效用。另2% GF對於正常飲食或是攝食高脂肪高膽固醇飲食的倉鼠可以減輕體重。2% GF、GD可以降低動物睪丸及腎周邊脂肪組織的重量。由實驗結果顯示，2% GF、GD可調節代謝、體脂肪平衡，而達到維持體重(抑或減重)的結果。未來可以應用在減少脂肪堆積抗肥胖、預防或治療代謝症候群之天然植物來源。

關鍵字

代謝症候群 ； 脆江蘺 ； 紅藻 ； 高脂血症

並列摘要

The metabolic syndrome represents some potential pathogenic factors and is often associated with high risk of cardiovascular disease, cerebrovascular disease, heart disease, diabetes and high blood pressure and other chronic diseases. Department of Health announced that among the top ten leading causes of death in Taiwan in 2011, six is associated with metabolic syndrome. It’s known that the accumulation of visceral lipid due to hyperlipidemia is the main reason causing the metabolic syndrome and excessive accumulation of lipid in our body is also linked to obesity, so the development of drugs or natural extracts with no side effects to reduce lipid in liver or human body has become the aim of researches to prevent or cure metabolic syndrome and obesity currently. Gracilaria burse-pastoris, one kind of seaweed plants, is currently the largest domestic production of algae and which is one kind of red algae. Some clinical reports have confirmed that the red algae play a role in the regulation of lipid metabolism while the mechanism of regulation of lipid metabolism from Gracilaria is not very clear. So our study is aimed to find out how Gracilaria fresh products (GF) and Gracilaria dry ones (GD) work to reduce blood lipid and lipid deposition in obese animal models. By adding 10% corn oil and 0.2% cholesterol in animal model to induce animal obesity in a period of eight weeks, we observed the changes of liver functions (ALT and AST), blood lipid values (TCHO, TG, HDL, LDL) and measured the weights of liver, testis and peripheral adipose tissue of kidney in each experimental group. In our preliminary observation in this animal model, we can’t conclude that GF or GD has significant effectives in the inhibition production of blood lipid while 2% GF do make hamsters lose weight either in normal diet or high-fat and high-cholesterol diet, and 2% GF and GD do reduce the weights of the animal gonadal and of renal peripheral adipose tissue. According to our study results, 2% GF and GD play a role in adjusting metabolism and balancing body lipid to maintain body weight (or help to lose weight). Maybe it can be used as the natural botanic source to reduce lipid deposition and anti-obesity and to prevent or treat of metabolic syndrome in the future.

並列關鍵字

Metabolic syndrome ； Gracilariaceae ； Rhodophyta ； Hyperlipidemia

參考文獻

農業統計年報. (2000). 行政院農業委員會出版.

許珊菁. (2005). 鼠模式中高脂飲食、肥胖與脂質調控基因之表現. 臺

黃瑞珉 (2005). 養殖廢污以龍鬚菜充分利用型之循環水系統設計. 臺

黃娣儀. (2000). 綠藻對於大白鼠體內脂質代謝的影響. 台北醫學院保

楊素卿. (2001). 綠藻對於餵食高油脂高膽固醇飼料之大白鼠脂質代謝