- 學位論文
薄型化塑膠微電泳質譜晶片之研究與發展
The development and study of polymeric thin-film electrophoresis microchip for Mass Spectrometry
摘要
微小化的分析晶片已成為現今一重要的研究方向；微小化可使得分析物消耗量及分析時間大幅減少，對醫療檢驗、基因分析、新藥開發等分析可說是十分重要。本研究之塑膠薄層電泳晶片，採用切割的方式切割出微管道，再配合護貝技術做晶片接合工作；將原來三度空間製程降低成兩度空間，在製作晶片時完全不需要考慮到深度問題，有簡化製程、大幅縮短晶片製作時間、以及降低製作成本之優點。 在本研究中，我們嘗試了塑膠薄層電泳晶片之微管道製作，以電腦切割與軌道式刀片切割方式，提升了微管道的精密度與再現性。接著我們利用塑膠薄層晶片具有剪裁容易之優點，直接採取在微管道出口端作裁剪來製作一體成形之質譜微電灑界面，大大的降低製作的難度。我們發展出具有極微小泰勒錐電噴灑之斜口尖型與側邊噴灑的斜口尖型質譜晶片；其中，側邊噴灑的斜口尖型質譜晶片，在製作上較簡單方便，只需在平頭管道旁做裁尖和斜切，便可製作出微電灑法噴嘴。最後以上述的塑膠薄層質譜晶片，改裝成脫附電灑游離法，來進行直接分析之研究，並應用於濫用藥物尿液、毛髪檢測與蔬果農藥殘留分析上。
關鍵字
微電泳質譜晶片並列摘要
The miniaturization of analysis chip has already become a new research trends; Indeed, such devices allow decreasing both the consumption of analytes and the duration of analyses for medical analysis, gene analysis, new medicine development etc, and also very important for the researches above-mentioned. In this study, we have developed a novel process for polymeric thin-film electrophoresis microchip. The micro-channel is fabricated by micro-blade, and then sealed by lamination without considering the depth of micro-channel. It reduces manufacture process from the 3-dimention to 2-dimention in CE chip fabrication. By this way, we take advantage of simply fabrication process and less fabricating time and cost. In addition, we made more improvement of the micro-channel fabrication by used the computer assisted mechanical tools. We developed the nano-electrospray ionization (ESI) chip for mass spectrometry by directly cutting out in the micro-channel end. By the way, it seems to reduce the process difficulty, and the nano-ESI chip formed very stable micro-Taylor core. Two type nano-ESI chip: beveled edge and the side beveled edge were developed and showed much better sensitivity。Moreover,the side beveled edge nano-ESI chip take advantage of simply fabrication process, only need to cut a sharp nozzle with the truncate channel end side . With above mentioned, we use the nano-ESI chip to develop the Desorption nano-electrospray ionization source (nano-DESI). The application for drugs of abuse in urine and hair, and for the residues of pesticides on the surface with fruit and vegetables were tested under LCQ ion trap mass spectrometry.
並列關鍵字
microchip參考文獻
延伸閱讀
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