柑橘果皮與果絡含水乙醇萃取物抗氧化、抗發炎及抑制幽門螺旋桿菌生長之研究

Antioxidation, antiinflammation and growth inhibition of Helicobacter pylori by aqueous ethanol extract of orange peel and pith

林臻(Jhen Lin)

指導教授 ： 王進崑 ； 陳肅霖

中山醫學大學/健康管理學院/營養學系碩士班/碩士(2010年)

https://doi.org/10.6834/CSMU.2010.00096

摘要

柑橘類水果佔全世界水果產量的大宗，已知柑橘類水果具有高抗氧化性及生物活性，包括抗發炎、抗心血管疾病、抗癌、抗菌等功效，已受到高度關注。柑橘果皮中含有豐富的類黃酮化合物，但果皮並不被食用，介於果皮與果肉間的網狀果絡，僅知主要含纖維質，但對其組成及可能之功效卻未曾被探討。因此本研究中取用台灣常見的椪柑及柳丁，分離其果皮及果絡的部份分別取得 70%乙醇水溶液萃取物進行抑制幽門螺旋生長及抗發炎之相關試驗。結果顯示，果絡萃取物同果皮具有抗氧化性，且果絡萃取物亦可以有效抑制 LPS誘發RAW264.7 巨噬細胞 NO 之生成及 COX-2 蛋白之表現。此外，果皮與果絡皆能抑制幽門螺旋桿菌之生長。由本研究結果可知，攝食柑橘果食時若能同時食用果絡，對健康可能更有助益。

關鍵字

柑橘 ； 果絡 ； 類黃酮 ； 抗氧化性 ； 抗發炎性 ； 幽門螺旋桿菌

並列摘要

Citrus fruits are mass produced in the world. Citrus fruits showed high antioxidation and biological activities, including antiinflammation, anti-cardiovascular disease, anti-cancer, anti-bacteria and so on. Citrus fruits peel contained rich flavonoids, but peel is not edible. Pith between peel and pulp contained rich fiber. The composition and biological activities of the peel fiber was never investigated. In this study, 70% ethanol extract of the peel and pith of Ponkan and Liucheng were used for determining the biological activities and antiinflammation. Results showed that ethanol extract of fruit peel and pith both exhibited antioxidation. In addition, pith inhibited COX-2 protein expression on LPS-induced macrophages (RAW264.7). Extracts of peel and pith could inhibit the growth of Helicobacter pylori. Therefore, together use of citrus fruit pulp and pith could be much helpful to health.

並列關鍵字

Citrus ； fruit pith ； flavonoids ； antioxidation ； antiinflammation ； Helicobacter pylori ； COX-2

參考文獻

呂政澤(2008)，牛樟芝純化成分抑制幽門螺旋桿菌誘導人類胃上皮細胞的

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