人類博卡病毒結構蛋白獨立區域對於巨噬細胞的影響

The Effects of Human Bocavirus VP1 Unique Region on Macrophage

蕭元超(Yuan-Chao Hsiao)

指導教授 ： 徐再靜

中山醫學大學/醫學院/免疫學研究所/碩士(2010年)

https://doi.org/10.6834/CSMU.2010.00102

摘要

中文摘要
人類博卡病毒(Human Bocavirus; HBoV)是微小病毒科中會感染人類的第二株病毒，主要由殼體蛋白VP1及VP2和非結構蛋白NS1及 NP1所組成。殼體蛋白VP1與VP2有重疊的區域，而沒有重疊的區域則稱為VP1殼體蛋白獨立區域(VP1 unique region,VP1u)。VP1u全長有129個胺基酸，胺基酸序列具有一段PLA2 motif，屬分泌型PLA2 (sPLA2)。先前文獻已知微小病毒科中的人類微小病毒B19 (B19) 結構蛋白獨立區域（VP1u）具有一段PLA2 motif，其sPLA2活性與病毒感染能力及促進細胞發炎反應有關。然而人類博卡病毒VP1u的功能並不清楚，因此本研究利用HBoV-VP1u和B19-VP1u重組蛋白為材料，探討其分泌型磷脂質（sPLA2）對巨噬細胞（Macrophage）的影響。結果發現HBoV-VP1u之sPLA2的活性會促進基質金屬蛋白分解酶MMP9的活性及發炎性細胞激素(IL-6、GM-CSF)之mRNA的表現。但HBoV- VP1u重組蛋白相較於B19-VP1u重組蛋白之sPLA2活性、MMP9活性及發炎性細胞激素(IL-6、GM-CSF)之mRNA的表現較低，具有統計學上差異(P<0.05)。綜合以上可知人類博卡病毒HBo-VP1u可以促使巨噬細胞活化進而引起先天性免疫防禦機制的啟動，這些發現將可提供未來對於人類博卡病毒之預防感染及疫苗開發上的一些線索參考。

關鍵字

人類博卡病毒 ； 結構蛋白獨立區域 ； 巨嗜細胞

並列摘要

Abstract
Human Bocavirus (HBoV) is the second human pathogen in the family of Parvoviridae. HBoV has three open reading frames that encode two nonstructural proteins, NS1 and NP1, and the structural proteins, VP1 and VP2. HBoV has two structural protein,VP1 (671 a.a.) and VP2 (542 a.a), which are identical except for 129 amino acids at the amino-terminal end of the VP1-protein, the so-called VP1-unique region (VP1u). Previously, a phospholipase A2 (PLA2) motif was identified in the VP1u and shown that B19-VP1u PLA2 motif can stimulate activation of macrophages. However, the effects of HBoV-VP1u PLA2 motif on initiating innate immunity by macrophages are still unknown. In this study, we found that the purified HBoV-VP1u proteins had sPLA2 activity. In addition, we found that VP1u-PLA2 motif induced the inflammatory cytokine expression, including IL-6 and GM-CSF by multiplex PCR. Moreover, we found that VP1u-PLA2 motif induced the MMP-9 expression. These findings may offer some clue in understanding the effects of macrophage on HBoV infection and for vaccine development in the future.

並列關鍵字

Human Bocavirus ； VP1 Unique Region ； Macrophage

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