- 學位論文
七層塔水萃取物保肝功能之評估
Hepato-protective effect of aqueous extract of Ocimum gralissimum L.
摘要
過去的實驗中證明七層塔水萃取物有著抑制肝臟星狀細胞活化的功效，接著進行動物試驗觀察七層塔的保肝作用。首先透過動物急毒性測試，餵食大鼠一到十五倍七層塔水萃取物後，血清中ALT、AST、ALP、ALB及LDH活性與正控制組並無差異性，顯示七層塔是安全無慮的。接著進行研究七層塔水萃取物是否具有抑制肝纖維化之功效，經過七層塔處理能使四氯化碳所誘導的肝臟細胞型態回復，細胞間質膠原蛋白堆積減少，血清ALT、AST活性下降，並抑制肝臟脂質過氧化生成，同時回復抗氧化酵素catalase活性和GSH的含量，進行西方墨點法發現，同時處理七層塔後，明顯抑制四氯化碳誘導肝臟內α-SMA蛋白表現。另外為了證明七層塔內有活性成份是否與多酚和類黃酮有關，更進一步純化七層塔水萃取物，並利用動物模式研究七層塔純化物短期保肝作用及抑制肝臟纖維化的功效。實驗發現，連續給予七層塔水萃取物七天，再利用單一四氯化碳造成肝臟受損，結果顯示在處理七層塔純化物後，可以預防肝臟細胞產生型變，抑制脂質過氧化物的產生和預防抗氧化酵素流失，證明了七層塔純化物有預防肝臟受損的功效。在四氯化碳誘導大鼠肝臟纖維化動物模式中，發現七層塔純化物也有著抑制肝臟纖維化的功效。綜合以上結果證明七層塔水萃取物和其純化物能預防肝臟發炎及抑制肝臟纖維化。
關鍵字
七層塔並列摘要
In the previous study, we demonstrated that Ociumu gratissimum L. could inhibit HSC activation. In this study, we investigated its hepteprotetive effects in vivo. First, we determined the toxicity of Ociumu gratissimum L.. After feeding 1 to 15 fold Ociumu gratissimum L., the level of serum of ALT、AST、ALP、ALB and LDH was no change. Therefore, we suggested that Ociumu gratissimum L. is safety in vivo. Furthermore, we derermined the effect of Ociumu gratissimum L. on the CCl4-induced fibrosis. That result showed that the extract of Ociumu gratissimum L., inhibited the CCl4-induced liver fibrosis, the elevated level of serum of ALT and AST, the lipidperoxydation production, and the collagen accumulation. In Western blotting assay, extract of Ociumu gratissimum L. also inihibited α-SMA expression in CCl4-induced liver fibrosis. In order to understand whether the polyphenol and Flavonoid of Ociumu gratissimum L. are concected with the protective effect, they were purified from the Ociumu gratissimum L.. The purified Ociumu gratissimum L. was given for 7 days, and then induced liver damage by treatmeant of CCl4 one time. After 24hr, the level of serum ALT、AST、lipid-peroxyidation and catalase activity were determined. The result show that the purified Ociumu gratissimum L. prevented hepatic cell metamorphosis, inhibited lipidperoxydation expression and reduced the consumption of anti-oxidase. Moreover, the purified Ociumu gratissimum L. also inhibited the CCl4-induced liver fibrosis. Therefore, these data suggested that the extracted and purified Ociumu gratissimum L. had the protective effects on anti-inflammtion and anti-liver fibrosis.