探討Skp2及p27在白血病、淋巴瘤患者的表現模式

Study the expression pattern of Skp2 and p27 in leukemia and lymphoma patients

沈建中(Chien-Chung Shen)

指導教授 ： 許立松

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2007年)

摘要

細胞週期負調節蛋白p27kip1（抑制癌症基因）主要的作用在於它可和Cdk2結合並抑制Cdk2的活性使細胞週期停留在G0到G1時期。許多的腫瘤細胞已證實p27kip1的表現會喪失，例如：乳癌及前列腺癌。臨床研究顯示在許多的腫瘤中的p27kip1的表現量會降低，例如：胃癌、肝癌、乳癌及前列腺癌且p27kip1的表現與其預後有很大的關係。細胞週期S協同性蛋白（S-phase kinase-associated protein 2, Skp2）是屬於F-box蛋白家族的成員之一，Skp2可藉由控制p27kip1來調節G1到S時期的進行，大量表現Skp2可以促進細胞週期的進展而且在老鼠身上會有淋巴癌的產生。在此研究，我們收集了32位白血病及31位淋巴瘤進行組織免疫染色，結果顯示在腫瘤組織中Skp2有較大量表現並伴隨著p27kip1表現量下降趨勢。

關鍵字

細胞週期S協同性蛋白 ； 抑制癌症基因 ； 白血病 ； 淋巴癌

並列摘要

The CDK inhibitor p27kip1 was initially discovered because it bound to and inhibited Cdk2 then induced cell cycle arrest at G0/G1 phase. Emerging reports have been demonstrated that loss or decrease in the level of p27kip1 protein is common in many human cancers such as breast cancer and prostate cancer. Low p27kip1 levels are an independent prognostic factor in gastric cancer, hepatocelluar carcinoma, breast cancer, and prostate cancer. The F-box protein, S phase kinase associated protein 2（Skp2）regulates G1-S transition by controlling p27kip1.Overexpression of Skp2 can trigger cell cycle progression and form lymphoma in transgenic mice. In this study, we have performed immuneohistochemical analysis of 32 leukemia and 31 lymphoma specimens. Our result have revealed that elevated expression of Skp2 was found in malignant part and combined with decreasing of p27kip1 expression.

並列關鍵字

Skp2 ； p27kip1 ； leukemia ； lymphoma

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