正常與代謝症候群成人血清中的Hedgehog蛋白濃度與年齡、性別、體位指標、生化值之比較

Comparison of Serum Hedgehog Protein Levels in Healthy and Metabolic Syndrome Populations with Age, Gender, Anthropometric, and Biochemical Parameters

李宜蓁(Yi-Chen Li)

指導教授 ： 林培正

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2008年)

摘要

Hedgehog (Hh) 訊息傳遞在動物發育過程中扮演著非常重要的角色。近來研究發現 Hh 在類脂肪細胞之分化過程中，會抑制脂肪細胞的分化特性。而且能抑制哺乳動物的脂肪生成，進而減少體內脂肪堆積。本研究利用林新醫院所提供的成人健康檢查個案，新陳代謝及肝膽腸胃科門診病患共 284 位的檢體進行分析，探討正常與代謝症候群人口血清中 Hh 蛋白濃度與年齡、性別、體位指數、生化值之比較是否有差異。研究方法包括理學、生化、免疫、腹部超音波等檢查、及血清 Hh 蛋白濃度定量共五大類。實驗結果發現，以 ELISA 方法分析血清或血漿中 Hh 蛋白濃度並無顯著性差異 (P> 0.05)，性別對於 Hh 蛋白濃度並無影響，而在 BMI ≧ 35的肥胖者發現血清中的Hh 蛋白濃度明顯比正常體位者低 (P< 0.001)。再以年齡超過或低於50歲分為兩個族群分析後發現 Hh 蛋白濃度隨年齡增加而減少 (P=0.0015)。同時統計結果顯示採樣的健康成人血清中的Hh 蛋白濃度的參考區間落在 4.4~181.3 pg/mL。另外在健康族群與糖尿病、脂肪肝患者比較發現健康族群50歲以下血清中 Hh 蛋白濃度高於糖尿病 (P<0.0001)、脂肪肝患者 (P< 0.001)。這些結果支持當 Hh 蛋白濃度低下時，可能導致脂肪之生成進而堆積引發代謝症候群的發生。

關鍵字

Hedgehog 蛋白 ； 脂肪形成 ； 參考區間 ； 代謝症候群

並列摘要

The hedgehog signaling pathway plays a key role during embryonic development. Moreover, in both cell culture and animal models, Hedgehog (Hh) protein was found to have the ability to suppress fat formation. The aim of this study was therefore to evaluate whether the serum level of Hh protein was altered, and subsequently influenced the adipose deposit between healthy and metabolic syndrome subjects. This experiment was designed to test by using human serum sample supplied by Lin Shin Hospital. A total of 284 samples were from Division of Metabolism and Division of Gastroenterology. Method included physical, biochemical, immunology examination, abdominal ultrasound, and Hh protein concentration quantification. The results showed that by using Enzyme-Linked Immunosorbent Assay (ELISA), Hh protein concentration demonstrated no difference between blood serum and plasma (P>0.05), and there was no difference between gender. Significantly lower Hh protein levels were found in the obese subjects with BMI ≧35, than those with normal anthropometric indices (P< 0.001). But there was difference between age group under and over 50, Hh protein concentration decreased accordginly while age increased. The reference interval of Hh protein concentration of health adult was within 4.4~181.3 pg/ml. In this experiment we also found, the Hedgehog protein concentration level of healthy group age under 50 was significantly higher than diabetes patients (P<0.0001) and fatty liver patients (P<0.001). Subjects with metabolic syndrome demonstrated lower concentration of Hh protein than the healthy ones. The result supports that lower Hh protein concentration may advance adipose deposit and subsequently induce metabolic syndrome.

並列關鍵字

Hedgehog protein ； Fat formation ； Reference interval ； Metabolic syndrome

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