蘋果多酚延緩脂肪細胞分化並在實驗性肥胖中降低脂肪貯存

Apple Polyphenols Decelerate Adipocyte Differentia-tion and Reduce Fat Storage in Experimental Obesity

林佩穎(Pei-Ying Lin)

指導教授 ： 李彗禎

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2010年)

摘要

肥胖已被認為具有造成糖尿病.心血管疾病及某些癌症等慢性疾病相關的高危險因子。先前的研究已知內臟中脂肪的堆積是造成代謝症候群的來源，如胰島素阻抗，與生活型態有關疾病如糖尿病、高脂血症及高血壓都會導致粥狀動脈硬化症的產生。於某些臨床報告中證實蘋果多酚具有調控脂質代謝。因此，於本研究中欲證實蘋果多酚於肥胖模式誘導下，其功效及機轉為何。細胞模式中，觀察蘋果多酚對於抑制3T3-L1細胞分化之影響。動物模式中，將倉鼠餵食高油脂飲食(含0.2%膽固醇及10%玉米油)誘導其肥胖，共為期八周，並觀察各組體重、肝臟重、腎臟周邊脂肪、性腺週邊脂肪、血清中三酸甘油酯、膽固醇、低密度脂蛋白及高密度脂蛋白。結果顯示，細胞模式中，蘋果多酚具有抑制3T3-L1細胞之分化能力。動物模式中，蘋果多酚可降低倉鼠體重及周邊脂肪重量。血清生化值結果中，蘋果多酚可降低血清中三酸甘油脂及膽固醇。以上的結果得知蘋果多酚具有降低脂質生成的能力。

關鍵字

蘋果多酚 ； 肥胖 ； 3T3-L1 ； 細胞分化

並列摘要

The global risk of overweight/obesity as well as the high incidence of diabetes, cardiovascular disease, and specific cancers has been recognized to associate with chronic diseases. Previous studies showed that visceral fat accumulation induces metabolic syndrome associated with insulin resistance, causing lifestyle-related diseases such as diabetes, hyperlipidemia, and hypertension, leading to arteriosclerosis. In some clinical studies reveals apple polyphenols (AP) can regulate fat metabolism in healthy subjects with high body mass index. In this study, we tried to clarify the effects and mechanisms of AP in an experimental obesity model. In in vitro study, the effect of AP inhibiting 3T3-L1 differentiation was investigated. In in vivo study, a high fat diet (0.2% of cholesterol and 10 % of corn oil) was fed to hamsters for 8 weeks to induce obesity. The results showed the AP reduced the level of 3T3-L1 cell differentiating to adipocyte. AP also reduced the body weight, pararenal and gonadal fat, the levels of triglyceride and total cholesterol in obese hamsters. . Our results show that AP possesses the ability to reduce the adipogenesis.

並列關鍵字

Apple polyphenol ； Obesity ； 3T3-L1 ； cell differentiation

參考文獻

許珊菁 (2005). "鼠模式中高脂飲食、肥胖與脂質調控基因之表現." 台灣大學微生物與生化學研究所.

Akazome, Y. (2004). "Characteristics and physiological functions of polyphenols from apples." Biofactors 311-314.

Ashrafi, K. (2007). "Obesity and the regulation of fat metabolism." WormBook 1-20.

Asma Ejaz, D. W., Paul Kwan, and Mohsen Meydani (2009). "Curcumin Inhibits Adipogenesis in 3T3-L1 Adipocytes and Angiogenesis and Obesity in C57/BL Mice." The Journal of Nutrition 919-925.

Berger, J. and D. E. Moller (2002). "The mechanisms of action of PPARs." Annu Rev Med 409-435.