- 學位論文
桑葉水萃物抑制酒精代謝乙醛預防急性肝損傷
Mulberry Leaf Extracts Prevented Acute Alcoholic Liver Injury via Reducing Acetaldehyde Production
摘要
飲酒過量會造成酒精性肝病，酒精透過肝臟代謝生成毒性產物乙醛(acetaldehyde)，此代謝物會傷害肝細胞導致肝臟發炎。桑葉已被證實具有抗氧化、降低血脂等功效，過去本實驗室曾證明在非酒精、化學性、細菌性誘導肝損傷模式下，桑葉水萃物(MLE ，Mulberry leaf water extract)具有減少肝損傷的效用，而桑葉對於急性酒精肝損傷的預防機理目前尚未釐清。本研究利用ICR小鼠評估桑葉是否具有改善酒精性誘導肝損傷模組中之功效，結果發現桑葉水萃物透過調節ADH(Alcohol dehydrogenase)、ALDH (Acetaldehyde dehydrogenase) 活性降低血清乙醛濃度進而減少肝毒性；此外，使用乙醇 (ethanol) 刺激肝臟細胞株的模式下，證實桑葉水萃物可抑制乙醇所導致發炎反應、細胞凋亡作用，並透過caveolin-1減少iNOS產生的氧化壓力(oxidant stress)。綜合以上結果，在急性酒精誘導下，MLE具有抑制乙醛生成之作用，降低肝細胞的發炎反應與氧化壓力，達到保肝目的。未來須進一步探討其護肝作用性成分，並期望發展為酒精肝損傷之護肝產品。
並列摘要
Alcoholic liver disease caused by excessive alcohol consumption. When liver processed alcohol metabolism, it would produce highly toxic chemicals acetaldehyde. This chemical could hurt the liver cells and then lead to inflammation. In previous studies demonstrated mulberry leaves had been not only possess anti-oxidant promoting effects, but also regulates serum fasting glucose and lipid metabolism. However, the effect of mulberry leaf extract (MLE) on reducing acute alcoholic liver injury is unclear. In this study, we have examined MLE effect on alcohol induced acute liver injury in ICR mice. We assayed alcohol /acetaldehyde dehydrogenases(ADH and ALDH) activities. We found that MLE could reduce serum acetaldehyde concentration and promote ALDH and inhibit ADH activation and reduce liver inflammation. In addition, we also verified MLE could reduce apoptosis and reduce iNOS oxidative stress via caveolin-1 in HepG2 cells line. In summary, our results show that MLE effectively prevented and caused the regression of experimental acute liver injury through reducing acetaldehyde production. In the future, we could find the way to do more research about finding more effective compounds from mulberry leave so that we would have more solution about treating liver disease.