用於骨組織工程的生物降解性矽酸鈣基複合骨水泥之體外物理化學性質

In vitro physicochemical properties of biodegradable calcium silicate-based composite cements for bone tissue engineering

薛乃碩(Nai-Shuo Hsueh)

指導教授 ： 丁信智

中山醫學大學/口腔醫學院/口腔材料科學研究所/碩士(2011年)

摘要

研究以溶膠凝膠法製備快速硬化且生物活性的矽酸鈣骨水泥，為提高其生物降解性與可注射性，於矽酸鈣中添加硫酸鈣與明膠微粒，製成生物降解性矽酸鈣基複合骨水泥，並探討此骨水泥用於骨組織工程之可行性。添加不同比例之硫酸鈣，其徑向拉伸強度為2.4-3.1 MPa，當硫酸鈣添加比例越高，硬化時間愈短，減少抗菌效果。添加明膠微粒，使材料具有抗沖刷性，但提高其硬化時間。於模擬體液中浸泡7天後，控制組（未添加硫酸鈣）之磷灰石沉積速率快於複合骨水泥。浸泡達180天後，未添加與添加60%硫酸鈣之組別，其重量損失分別為18%與38%，強度分別降低為1.6 MPa與0.98 MPa。添加硫酸鈣能提高矽酸鈣骨水泥之生物降解性，添加明膠微粒可以使矽酸鈣/硫酸鈣複合骨水泥具有抗沖刷性，此複合材料具有應用於骨組織工程之潛力。

關鍵字

矽酸鈣 ； 硫酸鈣 ； 明膠 ； 骨水泥 ； 骨組織工程 ； 生物降解性

並列摘要

The scaffold is the temporary supporting structure of tissue-engineered constructions. The objective of this study was to investigate the properties of calcium silicate/calcium sulfate/ gelatin composite cements for bone tissue engineering applications. The results showed that with the addition of calcium sulfate to calcium silicate, the increased diametral tensile strength values (2.4−3.1 MPa) and shortened setting time (7-25 min) of the cements were found; however, calcium sulfate resulted to the reduces antibacterial activity. Besides, the presence of gelatin microspheres appreciably improved the anti-washout capacity. When soaking in simulated body fluid (SBF) for 1 day, the precipitation of apatite particles for the control group without calcium sulfate was faster than the composite cements. After 180 days of soaking, weight loss for the cement specimens containing 0 and 60 wt% calcium sulfate were 18 and 38%, respectively. The corresponding diametral tensile strength values became 1.6 and 0.98 MPa after soaking for 180 days. Calcium sulfate could promote the degradation of calcium silicate-based cements and gelatin inproved anti-washout properties. The novel composite cement may be a potential candidate for bone tissue engineering.

並列關鍵字

Calcium silicate ； Calcium sulfate ； Gelatin ； Bone cement ； Tissue engineering, Biodegradation

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