- 學位論文
精油之細胞毒性、抗血小板凝集活性 及改善代謝症候群活性
Studies on the bioactivities of essential oils: cytotoxicity, the activity of anti-platelet aggregation, and the improvement on metabolic syndromes
摘要
本研究使用三十八種精油，包括來自臺灣的四種高緯度的樹木，紅檜、臺灣杉、柳杉和扁柏，以及三十四種商業上經常使用的精油，進行對五種腫瘤細胞的細胞毒性測定及抗血小板凝集試驗測定。 結果顯示，香柏精油和檀香精油對腫瘤細胞表現出明顯的細胞毒性。香蜂草和黑胡椒兩種精油對血小板凝集有中度抑制作用。本研究首次發現香柏和檀香對五種腫瘤細胞有明顯的細胞毒性。 本研究使用其中二十九種精油，包括三種不同來源的香蜂草(Melissa officinalis)精油，分析其在3T3 L1 脂肪細胞對葡萄糖消耗和脂肪累積上的影響。結果顯示香蜂草精油明顯的增加脂肪細胞對葡萄糖的消耗及減少脂肪的累積。此外，薄荷精油、薰衣草精油、佛手柑精油、香柏精油、白千層精油、玫瑰天竺葵精油、羅文莎精油被發現沒有增加葡萄糖的利用，但是在60 μL/L濃度時有抑制脂肪堆積的現象(和對照組比較約為65~91%之活性)。由於香蜂草精油有增加脂肪細胞對葡萄糖的利用及減少脂肪的累積作用，其作用途徑為AMPK/ACC路徑，檢測三種不同來源的香蜂草精油，以GC-MS比較其化學成分和生物活性，結果發現香蜂草精油含大量的檸檬醛混合其他的化合物，這些成分都可能在抗糖尿病作用上扮演重要的角色。
並列摘要
Thirty-eight essential oils (EOs), including 4 products from Taiwanese high-latitude trees, Chamaecyparis formosensis, Taiwania cryptomerioides, Cryptomeria japonica, and Chamaecyparis obtuse, as well as 34 commercial essential oils, were assayed on their cytotoxicity against 5 cancer cell lines and anti-platelet aggregation function. Two EOs from Juniperus virginiana and Santalum album showed significant cytotoxicity. Moreover, 2 EOs from Melissa officinalis and Piper nigrum exhibited moderate inhibitory effects on an anti-platelet aggregation assay. The cytotoxicity of EOs from J. virginiana and S. album was revealed for the first time. Twenty-nine commercial essential oils (EOs)including three different resources of Melissa officinalis essential oils, were assayed on their glucose consumption and lipid accumulation activities on 3T3-L1 adipocytes. The EOs of M. officinaliswere significantly active in both model assays. Moreover, EOs of Peppermint, Lavender, Bergamot, Cypress, Niaouli Nerolidol, Geranium-Rose and Revensara did not increase glucose consumption, but displayed inhibitory effect on lipid accumulation at 60 μL/L concentration (65~91% of lipid accumulation vs. the control 100 %). Because of the promising activity of M. officinalis EOs, three different products were collected and compared for their chemical profiles and bioactivity. The western blot data suggested that the key factors of the AMPK/ACC pathway can be mediated by M. officinalis EOs. Together with biodata, gas chromatography mass spectrometry (GC-MS) profiles suggested the mixtures of citrals and minor compounds of M. officinalis EOs may play an important role on the effect of anti-diabetes.