香菜萃出物對脂多醣體誘發小鼠RAW264.7巨噬細胞發炎反應之探討

The influence of Coriandrum sativum L. Extracts on LPS-induced inflammatory reactions in mouse RAW264.7 macrophage cells

巫玉琳(Yu-Ling Wu)

指導教授 ： 劉凱莉

中山醫學大學/健康管理學院/營養學研究所/碩士(2008年)

https://doi.org/10.6834/CSMU.2008.00103

摘要

香菜 (學名Coriandrum sativum L.) 是單年生草本傘形科植物，原產地在地中海沿岸及中亞地區，台灣各地均有栽培。香菜的莖和葉都有特殊的香味，普遍用來做為烹調時菜餚的點綴及提味之用。香菜性溫味甘，含有豐富營養素，其中以芳檸醇、維生素C、胡蘿蔔素、鈣等營養素含量最高。已有研究證實，香菜具有降血糖、降血脂、抗菌與抗氧化的作用，這些作用都與發炎反應有關，但香菜調控發炎反應的功效至今未知。巨噬細胞可分泌促發炎物質，造成組織細胞發炎，是身體免疫系統中重要防禦系統之一。被活化的巨噬細胞長期分泌促發炎物質而引起慢性發炎反應的發生，則與糖尿病、風濕性關節炎、癌症及心血管疾病等的發生具有密切相關性。本研究探討香菜 95% 乙醇萃出物對脂多醣體(lipopolysaccharide, LPS) 刺激小鼠RAW264.7巨噬細胞發炎反應之效應及相關調控機轉。結果顯示，單獨處理香菜95%乙醇萃出物或和LPS 10 ng/ml共同處理24小時，皆不影響存活率。處理香菜95% 乙醇萃出物(25-150 μg/ml)呈劑量關係的顯著抑制LPS誘發 inducible nitric oxide synthase、cyclooxygenase-2及pro-interleukin-1β之mRNA和蛋白質的表現與nitric oxide (NO)和 prostaglandin E2 (PGE2)生成(p<0.05)。另外，香菜95%乙醇萃出物可減少LPS刺激後IκBα磷酸化，抑制p65轉移至細胞核內，降低NF-κB與DNA結合之能力，而抑制促發炎基因表現。除此之外，香菜95%乙醇萃出物亦可降低LPS誘發Mitogen-activated protein kinases (MAPK)的蛋白質磷酸化表現。綜合以上結果得知:香菜可經由抑制LPS誘發轉錄因子NF-κB活化及MAPK訊息傳遞路徑，減少促發炎因子生成而具抗發炎之功效。

關鍵字

香菜 ； 小鼠RAW264.7巨噬細胞 ； 脂多醣體 ； 發炎反應

並列摘要

Coriandrum sativum L. is a local dietary plant commonly used in Middle eastern, Mediterranean, Indian, Southeast Asian. Coriandrum sativum L. has long been considered a fold remedy for various aliments in different cultures. A large number of components have been identified in Coriandrum sativum L., including flavonoids (quercetin and isoquercetin), polyphenols (rutin, caffeic acid derivatives), ascorbic acid and β-caroteinoids. Macrophages play a central role in the immune system which involves pathologic processes of several chronic diseases such as atherosclerosis, rheumatoid arthritis, diabetes and cancer. Activated macrophages can synthesize a serious of proinflammatory products to induce tissue inflammation. We examined the influence of Coriandrum sativum L.extracts on lipopolysaccharide (LPS)-induced inflammatory reactions in mouse RAW264.7 macrophage cells.Mitochondrial-dependent reduction of 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide assay showed that addition of different concentrations Coriandrum sativum L. extracts in the absence or presence of 10 ng/ml LPS treatment after 24 hours did not influence mouse RAW264.7 macrophage cell viability. Results indicated that Coriandrum sativum L. extracts dose-dependent inhibited LPS-induced protein and mRNA expressions of inducible nitric oxide synthase, pro-interleukin-1β and cyclooxygenase-2 as well as nitric oxide (NO) and prostaglandin E2 (PGE2) production (p<0.05). Coriandrum sativum L. extracts dramatically repressed LPS-induced IκBα phosphorylation and nuclear p65 protein expression as well as NF-κB nuclear protein-DNA binding activity. Moreover, Coriandrum sativum L. extracts reduced LPS-induced phosphorylated extracellular signal-regulated kinase 1/2, c-Jun N-terminal kinase 1 and p38 mitogen-activated protein kinase expression. These findings suggest that Coriandrum sativum L. through modulating NF-kB activation and mitogen-activated protein kinases signal transduction pathway suppresses the inflammatory responses in LPS-induced RAW264.7 macrophage cells.

並列關鍵字

Coriandrum sativum L. ； mouse RAW264.7 macrophage cell ； lipopolysaccharides ； inflammation

參考文獻

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