載入纖維母細胞生長因子(FGF-2） 的介孔洞矽酸鈣對於誘發人類牙髓細胞齒源性分化之效果

Enhanced Capability of fibroblast growth factor–loaded Mesoporous Calcium Silicate Scaffolds to Induce Odontogenic Differentiation of Human Dental Pulp Cells

陳曦(Hsi Chen)

指導教授 ： 黃翠賢

中山醫學大學/口腔醫學院/口腔科學研究所/碩士(2020年)

https://doi.org/10.6834/csmu202000160

摘要

在本研究中，我們使用不同濃度的纖維母細胞生長因子（FGF-2）載入介孔洞矽酸鈣材料並利用3D列印來印出支架。並觀察分別對於誘發人類牙髓細胞齒源性分化之效果是否有差異。實驗結果顯示，在支架表面結構部分，控制組與實驗組呈現相同的的表面結構。而在材料應力方面，三者的應力相似，並無統計上的差異。代表在加入纖維母細胞生長因子之後，並不會對於材料本身造成物理上的變化。在FGF2的釋出量方面，在兩者相比之下，則是在較高濃度的MCSF10組釋出量較高。在材料表面的生成物質在分析，控制組與實驗組所生成的表面物質均呈現有相同的結構，分析顯示為氫氧基磷灰石。此代表並不會因為添加MCSF影響纖維母細胞產生不同的礦化物表現。細胞生長分析，在培養天七天之後實驗組與控制組有明顯的細胞生長差異。而FGF受體細胞的部分，在高濃度這組有明顯的表現。鹼性磷酸酶的表現觀察，在細胞培養至天數14天，實驗組與控制組有呈現統計上的顯著差異。在動物實驗Micro CT 觀察支架之間骨頭生長， 實驗組的骨體積與骨密度是明顯高於控制組的。結語：添加了纖維母細胞生長因子後的介孔洞矽酸鈣支架，對於人類牙髓細胞的生長與骨分化效應有增加的效果，此改質之材料日後也許可以成為骨再生醫療選擇的參考材料。

關鍵字

介孔洞矽酸鈣 ； 纖維母細胞生長因子 ； 骨再生工程 ； 3D立體支架

並列摘要

In this study, we used different percentage of fibroblast growth factor loaded on Mesoporous Calcium Silicate Scaffolds using 3D printing techniques. MCS scaffolds are know for it properties such as inducing bone growth and bone regeneration. As fibroblast growth factor is quite known for tissue repair and cell growth, therefore we loaded fibroblast growth factor on Mesoporous Calcium Silicate hoping to gain a even better bone growth and wondering if there is any significant difference in inducing Odontogenic Differentiation of Human Dental Pulp Cells . The result shown that in all group, there’s no difference in tensile strength and also structure, in other words, that’s telling us there’s no excess materials in basic structure even after loaded FGF-2. FGF-2 also show’s that it plays an important role in increasing the rate of bone remodeling, bone tissue growth, and density of bone regeneration. In conclusion, this research indicated that loaded FGF enhanced the bone regeneration in MesoCS scaffold

並列關鍵字

MesoCS ； Fibroblast growth factor ； Bone regeneration ； 3D printed scaffold

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