桑葉功能性成分抑制第二型糖尿病腎臟病變機轉之研究

Mulberry leave extracts inhibit type IIdiabetic nephropathy

溫柏瀚(Bo-Han Wen)

指導教授 ： 張浤榮 ； 黃惠珮

中山醫學大學/醫學院/醫學研究所/碩士(2013年)

https://doi.org/10.6834/CSMU.2013.00210

摘要

背景:
糖尿病腎病(DN)是末期腎臟病變(ESRD)的單一主要原因，而有8% ~ 10%第二型糖尿病患者會罹患糖尿病性的腎臟病變，在這裡，我們設計這項研究，使用桑葉水萃取液，一種作為抗糖尿病的中國傳統天然藥物去研究對於糖尿病腎臟病變的發病機制。
材料與方法:
細胞方面使用小鼠的mesangial cell (MES 13 cell line)，購買自食品工業研究所生物資源保存及研究中心，使用桑葉水萃取液(MLE)進行抑制細胞增生試驗與細胞毒殺試驗以及西方墨點法實驗。
動物方面使用C57BL/6品系老鼠，給予100mg/Kg的STZ與240mg/Kg的NA後合併使用高脂質飼料餵食，並於每天給予桑葉水萃取液飲食含量1%和3%管餵，每兩週測量一次體重並進行葡萄糖耐受性測試、胰島素耐受性測試與血液生化值試驗。八週後，將小鼠犧牲，進行H.E. stain、PAS stain和Masson trichrome stain 與西方墨點法實驗。
結果與討論:
實驗結果顯示在細胞實驗方面，桑葉水萃取物確實可以抑制MES 13細胞增生的情形。而在動物實驗方面，誘導後給予桑葉水萃取物組別不管在OGTT、ITT還是各項血液生化值都改善之情形，因此可以推估桑葉水萃取液具有改善或減緩第二型糖尿性腎病變之效果。

關鍵字

桑葉 ； 糖尿病性腎臟病變

並列摘要

Backgrounds:
Diabetic nephropathy is a major cause of End-stage renal disease and the mortality rate due to this disease is continuously progressing worldwide. 8 to 10% patients of type 2 diabetes will suffer from diabetic nephropathy. Here, we design this study to elucidate the role of mulberry leave extracts (MLE), a traditional Chinese medicine as an anti-diabetic agent, in the pathogenesis of DN.

Materials and Methods:
The mouse cell aspect mesangial cell (MES 13 cell line) was purchased from the Food Industry Research Institute Biological Resources Conservation and Research Center. The cellswere treated with the mulberry leaf aqueous extract (MLE) in high glucose condition, andthe inhibitory effect of MLE cell proliferation induced by high glucosewas assayed by Western blot.
100mg/Kg streptozotocin (STZ) and 240mg/Kg nicotinamide (NA) were administered to C57BL/6J mice with high fat diet and 1%, 3% of MLEs. The plasma glucose concentration, body weight, oral glucose tolerance, insulin tolerance and albuminuria were monitored every two weeks. After 8 weeks later, mice were sacrificed and the kidneys were assayed by H.E.,PAS and Masson trichrome staining.
Resultsand Conclusion:
The results from the oral glucose tolerance (OGTT) and insulin resistance (ITT) tests showed that MLE decreased the plasma glucose and improved the insulin sensitivity in type II mice compared with the native group,indicating that MLEs ameliorated the renal atrophy in DM mice. In addition, diabetes-caused glomerular atrophy was recovered after treatment with MLEs in diabetic mice. Besides, MLE improved the MES-13 cells proliferation and ECM synthesis enhanced by high ambient glucose.Our results demonstrate that MLE has beneficial effects in diabetic nephropathy.

並列關鍵字

Mulberry leave extracts ； diabetic nephropathy

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