洛神花青素對3T3-L1脂肪細胞增生與脂質生成之調控作用

Modulatory actions of Hibiscus anthocyanins on cell proliferation and adipogenesis in 3T3-L1 adipocytes

邱珮舒(Pei-Su Chiu)

指導教授 ： 張雲菁

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2013年)

https://doi.org/10.6834/CSMU.2013.00214

摘要

肥胖為全球普遍之健康問題，是許多疾病的主要危險因子，例如:糖尿病、心血管疾病、高血壓及癌症等，然而，肥胖主要是由於脂肪量增加的結果，抑制脂肪細胞分化與脂肪細胞生成是目前評估抗肥胖之主要途徑。有相關的研究報告指出，天然植物富含之多酚類化合物已經證實具有抗氧化、抗發炎、抗癌、降低膽固醇等功效。因此，本研究希望從天然食物中尋找抗肥胖的成份，進而達到抗肥胖的效果。本研究萃取洛神花中富含的多酚類化合物-花青素(Hibiscus anthocyans，HAs)評估是否抑制脂肪細胞增生與脂質生成的調控作用。利用3T3-L1脂肪細胞之細胞模式，探討HAs對3T3-L1前脂肪細胞分化與分化過程中脂肪生成之影響。研究實驗包括：MTT assay、WST assay、PI stain/FACScan、Oil red O stain、胞內TG含量分析、western blotting等。結果證實，洛神花青素可有效抑制前脂肪細胞生長，減少分化過程中與分化後脂肪細胞的生長，並同時調控其相關蛋白質的表現，例如PPAR γ及FAS。因此，洛神花青素未來在預防及改善肥胖上應具有良好的潛力。

關鍵字

肥胖 ； 脂肪生成 ； 洛神花青素 ； 3T3-L1細胞

並列摘要

Obesity is a worldwide health problem, and it is a significant risk factor for various diseases such as diabetes, cardiovascular disease, hypertensive and cancer. However, obesity is mainly result of the increase volume due to fat, inhibit adipocyte differentiation and adipogenesis is the main way to evaluate anti-obesity. Related research report, rich in many natural plant phenolic compounds have been shown to have antioxidant, anti-inflammatory, anticancer and lower cholesterol properties. Therefore, this study hopes to find the compounds from food with anti-obesity ingredients, thus achieving the anti-obesity effect. One of the plants which are considered medicinal is Hibiscus sabdariffa Linnaeus with local name of Roselle. In this study, Roselle extract rich in polyphenols-anthocyanins, assess whether inhibition of adipocyte proliferation and the regulation of adipogenesis. Using the 3T3-L1 adipocytes cell model, we explored the effect of HAs (Hibiscus anthocyanin) on cell proliferation and adipogenesis in 3T3-L1 preadipocytes. Experimental studies include: MTT assay, WST assay, PI stain/FACScan, Oil red O stain, intracellular triglyceride accumulation assay and western blotting. These results suggest that HAs can effectively inhibit adipogenesis in 3T3-L1 preadiopocytes and adipocytes, also regulating the performance of PPAR γ and FAS。Therefore, Hibiscus anthocyanins may have potential for the treatment of obesity.

並列關鍵字

obesity ； adipogenesis ； Hibiscus anthocyanins ； 3T3-L1 adipocyte

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