土肉桂葉精油對於LPS誘發大鼠腸黏膜嗜中性球浸潤及發炎作用之影響

本研究目的為觀察灌食土肉桂精油(TC)是否可改善血管壁黏附相關因子和發炎激素含量及腸道組織嗜中性球浸潤。實驗於雄性Wistar大鼠分別灌食低、中、高劑量(13、26、53 mg/kgBw)TC或其主要成分肉桂醛(40 mg/kgBw)，為期兩週，共八次，然後腹腔注射LPS (5 mg/kgBw)誘發系統性發炎反應(Systemic inflammation response syndrome, SIRS)，並在注射後18小時犧牲。收集血液，分離嗜中性球，以流式細胞儀分析細胞表面CD11b、CD18表現量;以ELISA分析血中可溶性黏附因子sL-seletin、sICAM-1、趨化因子CINC-1及促發炎激素TNF-α、IL-1β和一氧化氮(NO)的濃度，並觀察迴腸道黏膜L-seletin、ICAM-1、CINC-1及TNF-α、IL-1β和NO的含量;嗜中性球浸潤腸黏膜組織之程度以迴腸組織中MPO活性及組織化學染色法觀察之，另以HE組織形態染色觀察腸道組織完整性。結果顯示，在不影響大鼠正常代謝下，所有劑量TC皆可顯著逆轉LPS誘發之嗜中性球表面黏附分子CD11b/CD18表現量，但肉桂醛並無效果；所有劑量TC及肉桂醛皆可顯著降低血清中sL-selectin、sICAM-1、CINC-1及NO含量，但在血清中促發炎激素方面，只有低劑量TC有著顯降低TNF-α含量，中劑量TC顯著降低IL-1β含量；在迴腸黏膜上皮組織之ICAM-1、CINC-1及TNF-α、IL-1β和NO含量上，則是所有劑量之TC組及肉桂醛組皆明顯低於LPS處理組；小腸MPO活性分析及MPO組織化學染色結果也顯示，TC可改善LPS造成的腸道嗜中性球浸潤現象，並有助於小腸黏膜完整性的維持，雖然肉桂醛改善腸道嗜中性球浸潤的效果不顯著，但仍能改善小腸黏膜完整性。由以上結果可推論，TC可經由減少血液嗜中性球數量、改善黏附和趨化因子濃度、改善腸黏膜促發炎激素含量，減輕LPS造成的大鼠腸黏膜嗜中性球浸潤程度，並且改善腸道絨毛的受損，且TC之抗發炎效果有部分與其成分肉桂醛有關。

關鍵字

嗜中性球；土肉桂；肉桂醛； LPS ；腸道；大鼠

並列摘要

The aim of the present study was to investigate the effect of leave essential oil of Cinnamomum osmophloeum Kaneh. on the infiltration activity of neurophils and the underlying mechanisms. Rats received every other day for 8 times by gavage low, medium or high dose of leave essential oil of Cinnamomum osmophloeum Kaneh. , Cinnamaldehyde (40mg/kg Bw), or vehicle (corn oil, 2 ml/kg Bw) followed by the injection with LPS (i.p., 5mg/kgBw) to induce neutrophil infiltration in the intestinal mucosa. The control group were rats received corn oil previously and injected with saline. At 18h after the injection, rats were killed with CO2 and biological samples were collected for various analysis. It showed that none of the animals affected by leave essential oil of Cinnamomum osmophloeum Kaneh. or cinnamaldehyde in term of their metabolism status. All tested doses of the essential oil but not cinammaldehyde significantly reversed LPS-induced elevation of CD11b/CD18 on neutrophil. In the other hand, all doses of the essential oil and cinnammaldehyde significantly lowered plasma levels of sL-selectin, sICAM-1, CINC-1 and NO. Nevertheless, it showed that only at low and middle but not high doses of the essential oil suppressed LPS-induced elevation of plasma levels of proinflammatory cytokines. For effects on local intestinal mucosa, all tested doses of the essential oil showed to be consistent with that of cinnamaldehye in terms of the reversal of LPS-induced elevation of ICAM-1, CINC-1, TNF-α, IL-1βand NO levels. It was found that the essential oil was able to reverse LPS-induced elevation of MPO activity in the intestinal mucosa and both the essential oil and cinnamaldehye were found to ameliorate the integrity of intestinal mucosal when investigated with HE staining. The results of the present study suggest that leave essential oil of Cinnamomum osmophloeum Kaneh. is able to reverse LPS-induced neutrophil infiltration and damage of the intestine at least via reducing peripheral neutrophil number, reducing peripheral and intestinal levels of adherion molecules and proinflammatory cytokines. Such effects of Cinnamomum osmophloeum Kaneh. is at least partly performed by cinnamaldehyde in this preparation.

並列關鍵字

neutrophil ； cinnamon ； cinnamaldehyde ； LPS ； intestine ； rat

參考文獻

Amalaradjou MA, Narayanan A, Baskaran SA, Venkitanarayanan K. Antibiofilm effect of trans-cinnamaldehyde on uropathogenic Escherichia coli. J Urol 184(1):358-363.

Anand P, Murali KY, Tandon V, Murthy PS, Chandra R. Insulinotropic effect of cinnamaldehyde on transcriptional regulation of pyruvate kinase, phosphoenolpyruvate carboxykinase, and GLUT4 translocation in experimental diabetic rats. Chem Biol Interact 186(1):72-81.

Atkins D, Furuta GT. Mucosal immunology, eosinophilic esophagitis, and other intestinal inflammatory diseases. J Allergy Clin Immunol 125(2 Suppl 2):S255-261.

Balzan S, de Almeida Quadros C, de Cleva R, Zilberstein B, Cecconello I. 2007. Bacterial translocation: overview of mechanisms and clinical impact. J Gastroenterol Hepatol 22(4):464-471.

Basset C, Holton J, O'Mahony R, Roitt I. 2003. Innate immunity and pathogen-host interaction. Vaccine 21 Suppl 2:S12-23.

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土肉桂葉精油對於LPS誘發大鼠腸黏膜嗜中性球浸潤及發炎作用之影響

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