利用糖尿病及血脂異常老鼠模式探討龍鬚菜萃取物對其副病變之作用

Effects of Sechium edule Shoot Extracts on Diabetes Mellitus, Diabetic Complications and Associated Lipid Metabolic Disorders in a rat model

邱瑀涵(Yu-Han Chiu)

指導教授 ： 王朝鐘 ； 張雲菁

中山醫學大學/醫學院/醫學研究所/碩士(2016年)

摘要

現行流行病學研究統計指出，多種危險因子與第二型糖尿病的發病有關，其中肥胖是一項最常見的因素，並且伴隨著代謝症候群的發生。根據實驗室之前的研究，龍鬚菜水萃物(water extracts of Sechium edule shoots, SWE)可以有效抑制油酸所誘導肝臟脂肪合成和脂肪分解增加。因此，本研究的目的是探討SWE對高油脂飲食（High fat diet, HFD）配合低劑量Streptozotocin (STZ)所誘發出的高血脂高血糖的糖尿病大鼠的抑制作用。將大鼠隨機分為4組：control組、HFD + STZ組、HFD + STZ + SWE0.5％組、HFD + STZ + SWE1.0％組。確認施打STZ之組別其血糖> 200 mg/dl後，即可開始加藥進行八週的試驗，於犧牲後將血清和組織進行分析。結果發現SWE0.5%及1.0%組的血糖有明顯比HFD + STZ組下降約20%。然而，血清中三酸甘油酯和及總膽固醇表現量並無受顯著影響。從組織切片做觀察，利用Hematoxylin-Eosin stain (H E stain)及油紅(Oil red O stain)染色分析，從加藥組與誘導組做比較，觀察到餵食SWE可明顯改善肝臟中脂肪泡的堆積。另外，腎臟組織利用H E、Periodic Acid-Schiff stain和Masson氏染色分析，結果表明，SWE亦可以避免由高血糖所引起的腎間質增生、醣質推積以及膠原蛋白增生。整體而言，本篇實驗證明在由高油脂飲食誘發之第二型糖尿病大鼠模式上，透過龍鬚菜萃取物的添加，可以穩定血液中葡萄糖的表現量，亦可以減緩高血糖所引起的副病變傷害，未來對於在代謝症候群的治療上亦有很大的幫助。

關鍵字

龍鬚菜 ； 糖尿病 ； 肥胖 ； 脂質代謝

並列摘要

Epidemiological studies indicate that obesity is important risk factors for several diseases, particularly type 2 diabetes. According to our previous study, the water extracts of Sechium edule shoots (SWE) inhibits oleic acid-induced hepatic lipogenesis and increases lipolysis. Therefore, the aim of this study is to investigate the anti-diabetic effects of SWE on high fat diet (HFD)-induced diabetes mellitus (DM). Sprague-Dawley (SD) rats were fed with HFD and then followed by low dose of streptozotocin (STZ) intraperitoneal injection result in type 2 diabetes. Rats were divided into 4 group: control group , HFD+STZ group , HFD+STZ+SWE0.5% group , HFD+STZ+SWE1.0% group. After completing the induction (mimic DM blood sugar level > 200 mg/dl), the animals were given 0.5% or 1% of SWE for further 8 weeks, and then the serum and tissue were collected to analyze. The blood glucose level in 0.5% and 1% SWE-treated groups were substantially reduced 20% as compared to the HFD+STZ group. The kidney tissues were analyzed by using Hematoxylin-Eosin stain, Periodic Acid-Schiff stain and Masson's staining. The results show that SWE decrease mesangial matrix expansion in the glomerular, accumulation of saccharide and collagen fiber expansion. Collectively, these data providence evidence that when supplemented SWE to a diet, can improve glucose homeostasis and improved insulin sensitivity in insulin resistant induced rats. This result suggests that Sechium edule shoots has potential to influence the development of metabolic syndrome.

並列關鍵字

Sechium edule shoots ； Diabetes mellitus ； Obesity ； Lipid metabolism

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