- 學位論文
用於雷射顯微摘取系統中電控平台之開發
Construction of a Electric Moving Stage for Laser Capture Microdissection System
摘要
本研究設計了新一代的雷射顯微摘取(Laser Capture Microdissection, LCM)系統之電控平台(Electric Moving Stage)。此電控平台的移動部份由步進馬達以及壓電致動器所構成，且為了改進系統的定位速度將光纖探針定位的自由度由三個減到一個，而讓顯微鏡載物平台擁有精密的二維移動自由度。並且透過Visual Basic 所撰寫出來的應用程式將電控平台與電腦人機介面的控制結合，且完成電動控制平台與雷射顯微摘取系統的整合。 本研究量測得到平台每間隔的位移量平均為3.265 μm，且經過統計學上的計算得到標準偏差值相對標準偏差值(Relative standard deviation, RSD)為0.00025，且實際地利用探針式雷射顯微摘取系統做測試，利用尖端針孔直徑為70奈米之光纖探針，成功地在EVA轉移薄膜上熱熔出所要的軌跡，本研究驗證了我們所開發出來的LCM系統電控平台定位的精密度高，可在一般雷射顯微摘取的實驗中，將視野中任意位置的目標細胞給摘取，且成功提升其定位的速度。
並列摘要
We construct a new Laser Capture Microdissection (LCM) system by Using Electric Moving Stage. It spends a lot of time in positioning when the new LCM system with near-field fiber probe is integrated with microscopy. For overcoming the shortcoming, we designed a precision stage for moving the sample to adjust the target to be in the center of the scope. The fiber probe was set over the view center, and allowed to move only up and down. The designed stage includes a stepping motor and piezo actuators for moving fine in X-Y directions. We build a component of Human Machine Interface on electric moving stage control system and LCM system by Visual Basic. Then, we actually use electric moving stage on LCM experiments. By statistical analysis, we obtained the average displacement value of the stage is 3.265 μm and Relative standard deviation(RSD) is 0.00025. We successfully melted the EVA transfer film into figure by using the 70 nm fiber probe. The target can be moved to the center of scope in one minute by this new stage, then the process of laser capture be completed more quickly and higher precision control.