纖維蛋白膠混合明膠顆粒應用於關節軟骨組織工程之研究

關節軟骨在我們的正常關節中扮演一個非常重要的角色，但由於其修復能力不佳，會使關節軟骨的修復相當困難，所以受損軟骨的修復就成為一個非常重大的課題。而天然材料中的纖維蛋白膠具有相當良好的生物相容性以及生物可降解性，並且在過去的研究之中，常以纖維蛋白膠作為細胞支架，用來促進軟骨組織的新生。另外，明膠不僅具有良好的生物相容性和生物可降解性，其還具有RGD序列，有利於細胞的培養和黏附。在本研究中，我們將製備明膠顆粒，並將其與纖維蛋白膠混合以作為軟骨組織工程研究中的細胞支架，且更進一步利用豬的軟骨細胞來評估此細胞支架運用於缺損關節軟骨修復的可行性。而由WST-1和LDH的測試結果顯示添加明膠顆粒的纖維蛋白膠具有很好的生物相容性。在葡萄糖胺聚醣（glycosaminoglycans, GAGs）定量試驗得知當細胞包覆於材料之中，能促進葡萄糖胺聚醣的持續分泌。而藉由Type II Collagen和MMP-1的基因表現可以證明添加明膠顆粒的纖維蛋白膠能更有利於軟骨細胞形態上的維持，並防止細胞外基質的分解。而最後更能由組織染色切片發現當細胞培養於含有明膠顆粒的纖維蛋白膠中，不但有利於軟骨細胞的生長和細胞外基質的分泌，更有助於軟骨細胞維持其原有的形態。可見此細胞支架能幫助軟骨細胞的生長，並有效維持其原有形態，應該能應用在組織工程中，以有助於關節軟骨的修復。

關鍵字

纖維蛋白膠；明膠；細胞支架；軟骨組織工程

並列摘要

Articular cartilage serves as an essential role in normal joint function. However, since the self-repair ability of damaged articular cartilage is poor, an artificial system of repairing damaged articular cartilage becomes a great challenge. Two potential components of scaffold have been selected in this work. Firstly, fibrin glue is studied because of its excellent biocompatibility and biodegradability. Another component, gelatin, has also been discussed because it is able to support cell culturing and adhesion. In this study, fibrin glue mixed with gelatin particles have been prepared to evaluate the possibility as the scaffold for articular cartilage tissue engineering. According to the measurement taken in vitro using WST-1 and LDH assay, the scaffold offers good biocompatibility. Glycosaminoglycans analyze shows that fibrin glue-gelatin particles would effectively promote extrancellular matrix (ECM) secretion. The gene expressions and histological staining demonstrate that the cells stayed in fibrin glue-gelatin particles could maintain chondrogenic phenotype and inhibit ECM degradation. The evidences mentioned above indicate that fibrin glue-gelatin particles would be a promising scaffold for articular cartilage tissue engineering.

並列關鍵字

Fibrin Glue ； Gelatin ； Scaffold ； Cartilage Tissue Engineering

參考文獻

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［3］ Buckwalter J.A., Mankin H. J., Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation. Instr. Course Lect. 1998; 47: 487-504.

［4］ Mansour J.M., Biomechanics of Cartilage, Lippincott Williams & Wilkins. 2004; 66-79.

［6］ Levangie P.K., Norkin C.C. and F.A. Davis, Joint structure and function :a comprehensive analysis 3rd ed. Phi- ladelpha, 2011; 49-83.

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纖維蛋白膠混合明膠顆粒應用於關節軟骨組織工程之研究

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