利用陽極處理法製備二氧化鈦奈米孔洞結構對類骨母細胞生物相容性之研究

鈦金屬及鈦合金廣泛應用於牙科及整形外科植入物，其材料表面特性將會影響植入後與骨細胞之生長，進而影響植體穩定度。本研究探討鈦金屬之表面處理方法，利用酸洗蝕刻處理提升表面粗糙度，在表面形成微米孔洞並利用陽極處理法製備奈米孔洞。藉由改變不同的製程參數，探討利用陽極處理法在不同條件下所得到的表面結構型態。實驗結果顯示，當陽極處理反應電壓越大、溫度越高、時間增長及氟化銨濃度的增加會影響奈米孔洞的生成速率及孔徑大小。當電壓上升，會使得表面奈米孔洞孔徑隨電壓升高而變大，隨著反應時間越長及反應溫度越高，皆會使得反應速率加快，可於表面得到更完整的奈米孔洞；但反應溫度及時間若再持續增加，將會導致表面氧化鈦會有破裂情形產生。陽極處理反應溫度控制在10℃，反應時間10分鐘，經FE-SEM觀察，可在表面得到奈米孔洞結構，且不會有破裂情形產生。將陽極處理實驗變因(反應溫度、時間、電位)經由實驗設計規劃，並與類骨母細胞MG-63培養於處理之試片上，探討細胞初期貼附形態、增生及生物相容性的影響。結果顯示，利用電化學陽極處理法能在短時間創造出奈米多孔性表面結構，在奈米孔洞孔徑 < 50 nm (18~46nm)其表面能提升類骨母細胞的增生情況並且擁有更佳的細胞貼附形態，進而能得到較佳的骨整合效果。

關鍵字

鈦金屬；陽極處理法；氧化鈦；奈米孔洞；類骨母細胞

並列摘要

Titanium (Ti) and Ti alloys are widely used as dentistry and orthopedic implants. The surface characteristics of the implant material can affect the osseointegration of bone cells and the long-term stability of the titanium implant. In this study, we applied an etching process to clean the implant surface and increase the surface roughness. Subsequently, various anodization parameters were implanted to create nanopores in different diameters on the implant surface. The associated surface morphology of the implants was determined by voltage, temperature, time and concentration of ammonium fluoride. The pore size increased as the voltage and temperature increased. However, if the time and temperature continued to increase, the surface of the titanium oxide tends to become fragile and ready to rupture. After the surface treatment, osteoblast-like cells were seeded onto treated materials to investigate the interaction between cells and implant materials. Design of Experiment was used to analyze the effect of factors including temperature, time and voltage on this interaction. The results indicated that osteoblast-like cells had better adhesion, proliferation and morphology on the nanoporous titanium surface (the pore size < 50 nm) than untreated or etched titanium. This study demonstrated that nanoporous surface structure of the titanium implant prepared by anodization treatment enhanced the osseointegration of the bone cells.

並列關鍵字

Titanium ； Anodic ； Titanium Dioxide Nanoporous ； Osteoblast-like Cell ； MG-63

參考文獻

1. Branemark, P.-I., et al., Intra-osseous anchorage of dental prostheses： I. experimental studies. Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, 1969. 3(2)： p. 81-100.

3. BRANEMARK, P.-I., Osseointegrated implants in the treatment of the edentulous jaw. Experience from a 10-year period. Scand. J. Plast. Reconstr Surg, 1977. 16： p. 1-132.

4. Adell, R., et al., A 15-year study of osseointegrated implants in the treatment of the edentulous jaw. International journal of oral surgery, 1981. 10(6)： p. 387-416.

5. Albrektsson, T. and L. Sennerby, State of the art in oral implants. Journal of clinical periodontology, 1991. 18(6)： p. 474-481.

6. Steinemann, S.G., Titanium—the material of choice? Periodontology 2000, 1998. 17(1)： p. 7-21.

國際替代計量

利用陽極處理法製備二氧化鈦奈米孔洞結構對類骨母細胞生物相容性之研究

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