新型極化生醫骨填充材料in vitro研究

In Vitro Study on the Effect of Polarization to the Addition of TiO2 to Calcium Phosphate Bio-Glass

梁竣昱(Chun-Yu Liang)

指導教授 ： 薛文景

國立臺北科技大學/工程學院/材料科學與工程研究所/碩士(2008年)

摘要

磷酸鈣形成生醫玻璃後，添加具奈米顆粒分佈之二氧化鈦使其具有類鈣鈦礦CaTiO3結晶陶瓷，而此結構的天然物或合成物有導電性。本研究研發新型極化生醫骨填充替代陶瓷進行In-Vitro 研究。使用射頻阻抗分析儀記錄材料經過電場極化第一至第八天後其介電性質的改變，探討其改變的機制，並對離開電場後材料極化持續時間進行量測。實驗運用X-ray繞射儀檢測材料極化前後晶體上之變化及檢測四點抗彎強度與密度再進行模擬人工體液浸漬實驗來模擬材料置於人體中之反應情形。最後進行In-Vitro 研究以MG-63成骨細胞株體外培養，檢測包括MTT細胞生長活性、鹼性磷酸酶細胞分化活性及掃描式電子顯微鏡表面型態觀察等。結果顯示，極化時間6天材料可達到飽合極化，即最大極化率。但極化時間超過4天之介電性質在極化持續時間的數據上並不理想，在離開電場後1天其介電性質即明顯大幅下降。材料極化前後在物理性質、機械性質及模擬人工體液（SBF）之研究結果差異甚小，但其可佐證此新型生醫材料確具有一定之機械強度及生物相容性。高介電常數性質之含鈦玻璃陶瓷骨替代材在細胞生長活性、鹼性磷酸酶活性數據方面皆優於未極化之骨替代玻璃陶瓷。因此判斷其具有較高之生物相容性並有助於成骨細胞生長。本研究可進一步了解材料應用在高應力之人體硬組織及生物相容性評估。

關鍵字

骨替代物 ； 磷酸鈣 ； 奈米氧化鈦 ； 極化 ； 介電性質

並列摘要

Titanium dioxide added to calcium phosphate bioglass to fabricate a piezoelectric biological ceramic (PVC) will produce a crystal structure of Perovskite. This study is developing a novel bone substitute from polarized PVC. By using RF impedance analysis instrument to record the effects of the dielectric constant on the materials polarization within electric field for 1 to 8 days, mechanism changes from measuring 4-point bending strength, density, crystal structure of X-ray diffraction, and duration polarized PVC will be deduced. The soak tests of PVC in simulation body fluid are performed as well. The MG-63 cell line and test includes MTT cell proliferation assay, alkaline phosphatase active, and SEM are obtained from In-Vitro studies. Maximum polarizability may be achieved by 6 days of polarization. However, duration of the 4 days of polarization surpassed and the dielectric constant begin to drop when taken out from electric field after 1 day. Physical and mechanical property together with soaking test in simulation body fluid (SBF) test show little difference from polarization, however, the evidence exhibits certainty of mechanical strength and biological compatibility. From the experimental result of the MTT cell proliferation assay, alkaline phosphatase active, and SEM observation, the polarized bioceramic with high dielectric constant will result a good cell proliferation and differentiation.

並列關鍵字

titanium dioxide ； piezoelectric biological ceramic（PVC） ； bone substitute ； polarization ； dielectric constant ； bone substitute ； tri-calcium phosphate

參考文獻

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