使用低成本CMOS影像感測器於DNA偵測之可靠度分析

Reliable DNA Detection by Low Cost CMOS Sensors

蔡宗達(Thung-Ta Tasi)

指導教授 ： 李達生

國立臺北科技大學/機電學院/能源與冷凍空調工程系碩士班/碩士(2010年)

摘要

熱對流聚合酶連鎖反應，主要是控制兩個加熱器溫度的不同，造成檢體溫度梯度使其產生熱對流現象之DNA增生技術。熱對流聚合酶連鎖反應，降低了控溫系統對DNA增生過程的影響，進而提高DNA檢測之可靠度。本論文所開發之螢光檢測儀器，透過熱對流聚合酶連鎖反應可成功增生DNA，並使用低成本之CMOS影像感測器與邊緣偵測其螢光，成功偵測HBV DNA 濃度103copies/mm、105 copies/mm及107 copies/mm，其成功偵測率分別為16.83%、83.33%及100%。其103copies/mm較低成功偵測率之可能原因為CPCR在增生103copies/mm 較不穩定，所以造成在螢光訊號之檢測成功率上偏低。
透過加速壽命測試驗證其螢光檢測儀器，在不需定期校正與維護之下，仍有一定是可靠度、DNA螢光偵測率。

關鍵字

聚合酶連鎖反應 ； 即時聚合酶連鎖反應 ； 互補式金屬氧化半導體感測器 ； 螢光偵測 ； 邊緣偵測

並列摘要

Convective Polymerase Chain Reaction(cPCR) mainly keep two different temperature to Make thermal convection phenomenon of HBV DNA in the tube.
cPCR has lower cost of thermal cycle controller. On the amplification processes of DNA, thus enhancing the reliability of DNA testing. In the study presented a new apparatus incorporated with a fluorescence detection module, cPCR can be successfully amplify DNA by using low cost CMOS sensors to detect the HBV DNA fluorescence with the edge of the successful detection of HBV DNA concentration of 103copies/mm, 105copies/mm and 107copies/mm, the percent of HBV DNA fluorescence detection was 16.83%, 83.33% and 100%. The low percentage of 103copies/mm of HBV DNA fluorescence detection is because the unstable of amplify the DNA fluorescence.
Accelerated lift testing was adopted to evaluate the reliability of the fluorescence detection apparatus. According to the life test plan, this proposed fluorescence detection apparatus was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the fluorescence detection apparatus, but also verifies the effectiveness of our systematic method for achieving a robust design.

並列關鍵字

Polymerase Chain Reaction ； PCR ； Real-time PCR ； CMOS sensor ； Fluorescence ； edge detection

參考文獻

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[23] 蔡宜璋，「影像感測元件產業」，TISC研究週報，台北，2008

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