本研究所使用的狼尾草是一種深具潛力的能源作物,經由酵素水解後,再進行微生物醱酵,可將其轉換為乙醇、琥珀酸等平台化學品(Platform chemicals)。本實驗室先以蒸煮爆碎法處理後的狼尾草渣,再經由鹼性過氧化氫處理法處理,開發酵素水解及醱酵的製程。本研究的重點在於鹼性過氧化氫預處理法(Post-Treatment)的最適化,探討鹼性過氧化氫反應濃度、時間、固液比對於木質素去除率以及糖化率的影響。 研究結果顯示,兼顧醣類回收率、反應時間等因素,在雙氧水濃度2%(wt/wt),固液比1:20,反應時間30 分鐘下,木質素去除率71.76%,而葡萄糖回收率與糖化率則分別可達84.98%和90.64% 此外,界面活性劑能夠穩定酵素,增加纖維素水解成可醱酵糖的產率。因此本研究添加不同種類、不同劑量的介面活性劑於水解製程中,探討界面活性劑對於狼尾草水解之效益。界面活性劑為Tween 80時,且劑量為4.5 g/L時,葡萄糖濃度可以達最高。
Napiergrass (Pennisetum Purpureum) is regarded as apotential energy crop, the napiergrass via enzymatic hydrolysis, follow by fermentation can be converted to platform chemicals, such as ethanol, succinic acid, etc. The residual solids after steam explosion were subjected to alkaline hydrogen peroxide treatment before enzymatic hydrolysis. The effects of alkaline hydrogen peroxide residence time and hydrogen peroxide concentration on lignin removal and enzymatic saccharification was studied. When saccharides recovery are considered, a milder alkaline hydrogen peroxide condition should be used, The results indicate that the highest yield of saccharides was the hydrogen peroxide concentration is 2 %(wt/wt), Solid-liquid ratio is 1:20 and the residence time is 30 minute, the lignin removal and enzymatic saccharification were 71.76 % and 84.98 %. In addition, surfactants could incrase enzyme stability and enhance the yield of cellulose to fermentationable sugars. Therefore, we add different types and different concentration of surfactants in the process of enzymatic hydrolysis to explore the effect of surfactant in the Napiergrass hydrolysis. When the surfactant is Tween 80, and a dose of 4.5 g / L, glucose concentrations can be up to the highest.